Abstract
Tilapia (Oreochromis niloticus) were exposed to 0, 100, 400, and 800 μg/g concentrations of Pb in diet for 60 days, and changes in growth performance, metabolic enzyme activities, and essential trace elements (Fe, Cu, and Zn) content in liver and kidney were investigated. Daily weight gain, feed conversation ratio, and survival of tilapia were not significantly affected by dietary Pb. Alanine transaminase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) activities in liver and kidney were affected by dietary Pb in a dissimilar way: Pb concentration-related decreases in ALT, AST, and LDH activities were observed in kidney, while these enzyme activities in liver were stimulated in a Pb concentration-dependent manner. It was demonstrated that the inhibitory effects of dietary Pb on alkaline phosphatase, Na, K-adenosine triphosphatase (ATPase), Ca, and Mg-ATPase activities in both liver and kidney were Pb concentration-dependent. It was also indicated that the content of Fe, Cu, and Zn in liver and kidney decreased with the increasing dietary Pb concentrations. The results suggested that long-term dietary Pb exposure could affect metabolic enzyme activities and the content of Fe, Cu, and Zn in liver and kidney, whereas growth impairment was not observed in tilapia.
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Weber DN (1993) Exposure to sublethal levels of waterborne lead alters reproductive behavior patterns in fathead minnows (Pimephales promelas). Neurotoxicology 14:347–358
Patel M, Rogers JT, Pane EF, Wood CM (2006) Renal responses to acute lead waterborne exposure in the freshwater rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 80:362–731
Alves LC, Glover CN, Wood CM (2006) Dietary Pb accumulation in juvenile freshwater rainbow trout (Oncorhynchus mykiss). Arch Environ Contam Toxicol 51:615–625
Blasco J, Puppo J (1999) Effect of heavy metals (Cu, Cd and Pb) on aspartate and alanine aminotransferase in Ruditapes philippinarum (Mollusca: Bivalvia). Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 122:253–263
Karan V, Vitorović S, Tutundzić V, Poleksić V (1998) Functional enzymes activity and gill histology of carp after copper sulfate exposure and recovery. Ecotoxicol Environ Saf 40:49–55
Haouem S, Hmad N, Najjar MF, El Hani A, Sakly R (2007) Accumulation of cadmium and its effects on liver and kidney functions in rats given diet containing cadmium-polluted radish bulb. Exp Toxicol Pathol 59:77–80
Lan WG, Won MK, Chen N, Sin YM (1995) Effect of combined copper, zinc, chromium and selenium by orthogonal array design on alkaline phosphatase activity in liver of the red sea bream, Chrysophrys major. Aquaculture 131:219–230
Atli G, Canli M (2007) Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus. Comp Biochem Physiol C Toxicol Pharmacol 145:282–287
Parvez S, Sayeed I, Raisuddin S (2006) Decreased gill ATPase activities in the freshwater fish Channa punctata (Bloch) exposed to a diluted paper mill effluent. Ecotoxicol Environ Saf 65:62–66
de la Torre FR, Salibián A, Ferrari L (2000) Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environ Pollut 109:277–282
Alves LC, Wood CM (2006) The chronic effects of dietary lead in freshwater juvenile rainbow trout (Oncorhynchus mykiss) fed elevated calcium diets. Aquat Toxicol 78:217–232
Vivante A, Irshoren HN, Shochat T, Merkel D (2008) Association between acute lead exposure in indoor firing ranges and iron metabolism. Isr Med Assoc J 10(4):292–295
Blanusa M, Piasek M, Kostial K (1989) Interaction of lead with some essential elements in rat's kidney in relation to age. Biol Trace Elem Res 21:189–193
White FD, Neathery MW, Gentry RP, Miller WJ, Logner KR, Blackmon DM (1985) The effects of different levels of dietary lead on zinc metabolism in dairy calves. J Dairy Sci 68(5):1215–1225
Fick KR, Ammerman CB, Miller SM, Simpson CF, Loggins PE (1976) Effect of dietary lead on performance, tissue mineral composition and lead absorption in sheep. J Anim Sci 42(2):515–523
Doyle JJ, Younger RL (1984) Influence of ingested lead on the distribution of lead, iron, zinc, copper and manganese in bovine tissues. Vet Hum Toxicol 26(3):201–204
Rogers JT, Richards JG, Wood CM (2003) Ionoregulatory disruption as the acute toxic mechanism for lead in the rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 64:215–234
Reitman S, Frankel S (1957) Colorimetric determination of glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:53–56
Tang Y, Cen L, Hu CB, Li C, Xu M, Luo Y, Lu C (2006) Purification and properties of alkaline phosphatase of silkworm Bombyx mori. Front Biol China 1:246–253
Zaheer N, Iqbal Z, Talwar GP (1968) Metabolic parameters of ontogenesis of electrical activity in the brain. Sodium–potassium activated adenosine triphosphatase in developing chick embryo. J Neurochem 15:1217–1224
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Velmurugan B, Selvanayagam M, Cengiz EI, Uysal E (2008) Levels of transaminases, alkaline phosphatase, and protein in tissues of Clarias gariepienus fingerlings exposed to sublethal concentrations of cadmium chloride. Environ Toxicol (in press)
Masola B, Chibi M, Kandare E, Naik YS, Zaranyika MF (2008) Potential marker enzymes and metal–metal interactions in Helisoma duryi and Lymnaea natalensis exposed to cadmium. Ecotoxicol Environ Saf 70:79–87
De Smet H, Blus R (2001) Stress responses and changes in protein metabolism in carp Cyprinus carpio during cadmium exposure. Ecotoxicol Environ Saf 48:255–262
Almeida JA, Diniz YS, Marques SF, Faine LA, Ribas BO, Burneiko RC, Novelli EL (2002) The use of the oxidative stress responses as biomarkers in Nile tilapia (Oreochromis niloticus) exposed to in vivo cadmium contamination. Environ Int 27:673–679
Adhikari N, Sinha N, Saxena DK (2000) Effect of lead on Sertoli–germ cell coculture of rat. Toxicol Lett 116:45–49
Antonio Garcia T, Corredor L (2004) Biochemical changes in the kidneys after perinatal intoxication with lead and/or cadmium and their antagonistic effects when coadministered. Ecotoxicol Environ Saf 57:184–189
El-Demerdash FM, Elagamy EI (1999) Biological effects in Tilapia nilotica fish as indicators of pollution by cadmium and mercury. Int J Environ Health Res 9:173–186
Boge G, Leydet M, Houvet D (1992) The effects of hexavalent chromium on the activity of alkaline phosphatase in the intestine of rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 23:247–260
Thaker J, Chhaya J, Nuzhat S, Mittal R, Mansuri AP, Kundu R (1996) Effects of chromium (VI) on some ion-dependent ATPases in gills, kidney and intestine of a coastal teleost Periophthalmus dipes. Toxicology 112:237–244
Ay Ö, Kalay M, Tamer L, Canli M (1999) Copper and lead accumulation in tissues of a freshwater fish Tilapia zillii and its effects on the branchial Na, KATPase activity. Bull Environ Toxicol 62:160–168
Cerklewski FL, Forbes RM (1976) Influence of dietary zinc on lead toxicity in the rat. J Nutr 106(5):689–696
Flora SJ, Singh S, Tandon SK (1989) Thiamine and zinc in prevention or therapy of lead intoxication. J Int Med Res 17(1):68–75
Cerklewski FL (1984) Postabsorptive effect of increased dietary zinc on toxicity and removal of tissue lead in rats. J Nutr 114(3):550–554
Cerklewski FL (1979) Influence of dietary zinc on lead toxicity during gestation and lactation in the female rat. J Nutr 109(10):1703–1709
Jamieson JA, Shuhyta JN, Taylor CG (2007) Lead does not affect transcription of intestinal zinc-binding proteins in growing rats. Exp Biol Med (Maywood) 232(6):744–753
Gasiorowski K, Pawlowski T, Spychala J, Swiatek J, Kozlowski H (1987) The influence of subcutaneously administered lead(II) acetate on the concentrations of copper, iron, and zinc in the blood, kidney, liver, and spleen of rats. Sci Total Environ 64(1–2):117–123
Petering HG (1978) Some observations on the interaction of zinc, copper, and iron metabolism in lead and cadmium toxicity. Environ Health Perspect 25:141–145
Klauder DS, Petering HG (1975) Protective value of dietary copper and iron against some toxic effects of lead in rats. Environ Health Perspect 12:77–80
Cerklewski FL, Forbes RM (1977) Influence of dietary copper on lead toxicity in the young male rat. J Nutr 107(1):143–146
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The financial support provided by Science and Technology Planning Project of Zhejiang Province (Grant No 2008C32034) and Educational Commission of Zhejiang Province (Grant No. N20080197) is gratefully acknowledged.
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Dai, W., Fu, L., Du, H. et al. Changes in Growth Performance, Metabolic Enzyme Activities, and Content of Fe, Cu, and Zn in Liver and Kidney of Tilapia (Oreochromis niloticus) Exposed to Dietary Pb. Biol Trace Elem Res 128, 176–183 (2009). https://doi.org/10.1007/s12011-008-8259-8
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DOI: https://doi.org/10.1007/s12011-008-8259-8