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Study of Antibacterial Activity of ZnZnbisporphyrin Complexes and its Free Components on Staphylococcus aureus by Microcalorimetry

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Abstract

The antibacterial effect of Zn(II), tetraphenyl porphyrin (TPP), propdioxyl bridged tetraphenyl bisporphyrin 1, and its metallobisporphyrin complexes (ZnMnbisporphyrin 2 and ZnZnbisporphyrin 3) towards Staphylococcus aureus growth was investigated by microcalorimetry at 37°C. Differences in their capacities to inhibit the growth metabolism of S. aureus were observed. By analyzing the power–time curves, crucial parameters such as the rate constant of bacterial growth (k), inhibitory ratio (I), and generation time (t G) were determined. The growth rate constant (k) of S. aureus (in the log phase) in the presence of the drugs decreased linearly with increasing concentrations of the complexes. The sequence of the antibacterial activities of these compounds tested was 3 > 2 > 1 > Zn(II) > TPP. ZnZnbisporphyrin 3 is proposed to benefit from the synergetic effects of Zn(II) and 1.

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Acknowledgments

We gratefully acknowledge the financial support of project supported by the National Natural Science Foundation of China (30570015), Creative Scientific Research Group Foundation (NSFC 20621502), Science Research Foundation of Chinese Ministry of Education ([2006]8IRT0543), and Natural Science Foundation of Hubei Province (2005ABC002). We thank the reviewers for their critical comments on the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072, China

    Xiang-jiao Xu, Chun-qiao Chen, Zhuo-yuan Wang, Yi Zhang, An-xin Hou & Yi Liu

  2. College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, China

    Xiang-jiao Xu

  3. School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei, 430072, China

    Chao-Hong Li

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  1. Xiang-jiao Xu
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  2. Chun-qiao Chen
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  3. Zhuo-yuan Wang
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  4. Yi Zhang
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  5. An-xin Hou
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  6. Chao-Hong Li
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  7. Yi Liu
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Correspondence to Yi Liu.

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Xu, Xj., Chen, Cq., Wang, Zy. et al. Study of Antibacterial Activity of ZnZnbisporphyrin Complexes and its Free Components on Staphylococcus aureus by Microcalorimetry. Biol Trace Elem Res 127, 269–277 (2009). https://doi.org/10.1007/s12011-008-8243-3

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  • DOI: https://doi.org/10.1007/s12011-008-8243-3

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