Abstract
The purpose of the present work was to evaluate the iron bioavailability of a new ferric pyrophosphate salt stabilized and solubilized with glycine. The prophylactic–preventive test in rats, using ferrous sulfate as the reference standard, was applied as the evaluating methodology both using water and yogurt as vehicles. Fifty female Sprague–Dawley rats weaned were randomized into five different groups (group 1: FeSO4; group 2: pyr; group 3: FeSO4 + yogurt; group 4: pyr + yogurt and group 5: control). The iron bioavailability (BioFe) of each compound was calculated using the formula proposed by Dutra-de-Oliveira et al. where BioFe % = (HbFef − HbFei) × 100/ToFeIn. Finally, the iron bioavailability results of each iron source were also given as relative biological value (RBV) using ferrous sulfate as the reference standard. The results showed that both BioFe % and RBV % of the new iron source tested is similar to that of the reference standard independently of the vehicle employed for the fortification procedure (FeSO4 49.46 ± 12.0% and 100%; Pyr 52.66 ± 15.02% and 106%; FeSO4 + yogurth 54.39 ± 13.92% and 110%; Pyr + yogurt 61.97 ± 13.54% and 125%; Control 25.30 ± 6.60, p < 0.05). Therefore, the stabilized and soluble ferric pyrophosphate may be considered as an optimal iron source for food fortification.
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References
Boccio J, Salgueiro J, Lysionek A, Zubillaga M, Weill R, Goldman C y Caro R (2003) Current knowledge of iron metabolism. Biol Trace Element Res 92:189–212
Bothwell T, McPhail P (1992) Prevention of iron deficiency by food fortification. In: Fomon SJ, Zlotkin S (eds) Nutritional anemias. Nestlé Nutrition Workshop series 30. Raven, New York, pp 183–192
Lysionek AE, Zubillaga MB, Salgueiro MJ, Caro R, Ettlin E y Boccio J (2001) Bioavailability studies of a new iron source by means of the prophylactic–preventive method in rats. Biol Trace Element Res 84:123–128
Bernard HJ (1988) Hematology and coagulation. In: Todd, Sanford, Davidsohn (eds) Clinical diagnosis and management by laboratory methods. 17th edn. Saunders, Philadelphia
Reeves PG, Nielsen FH, Fahey GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc Writing Committee on the reformulation of the AIN-76 rodent diet. J Nutr 123:1939–1951
Dutra-de-Oliveira JE, Freitas MLS, Ferreira JF, Gonçalves AL, Marchini JS (1995) Iron from complex salts and its bioavailability to rats. Int J Vit Nutr Res 65:272–275
Sokal RR, Rohlf FJ (1981) Biometry. Freeman, San Francisco
Salgueiro MJ, Zubillaga MB, Lysionek AE, Caro R, Weill R y Boccio J (2002) Fortification strategies to combat zinc and iron deficiency. Nutr Rev 60(2):52–58
Hurrel RF (2002) How to ensure adequate iron absorption from iron-fortified food. Nutr Rev 60:S7–S15
Wegmüller R, Zimmermann MB, Moretti D, Arnold M, Langhans W, Hurrel RF (2004) Particle size reduction and encapsulation affect the bioavailability of ferric pyrophosphate in rats. J Nutr 134:3301–3304
Rhoner F, Ernst FO, Arnold M, Hilbe M, Biebinger R, Ehrensperger F, Pratsinis S, Langhans W, Hurrel RF, Zimmermann MB (2007) Synthesis, characterization and bioavailability in rats of ferric phosphate nanoparticles. J Nutr 137:614–619
Fritz JC, Pla GW, Harrison BN, Clark GA, Smith EA (1978) Measurement of the bioavailability of iron, using the rat hemoglobin repletion test. J Assoc Off Anal Chem 61:709–714
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Salgueiro, M.J., Arnoldi, S., Kaliski, M.A. et al. Stabilized–Solubilized Ferric Pyrophosphate as a New Iron Source for Food Fortification. Bioavailability Studies by Means of the Prophylactic–Preventive Method in Rats. Biol Trace Elem Res 127, 143–147 (2009). https://doi.org/10.1007/s12011-008-8229-1
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DOI: https://doi.org/10.1007/s12011-008-8229-1