Skip to main content
SpringerLink
Log in

Antioxidant Capacity of Flavonoid in Soybean Seedlings under the Joint Actions of Rare Earth Element La(III) and Ultraviolet-B Stress

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

The dynamic state of antioxidant capacity of flavonoid was investigated for a further demonstration of alleviating the damage of the ultraviolet (UV)-B radiation in the La-treated soybean seedlings under UV-B stress. Using hydroponics culture, the effects of lanthanum on the contents of flavonoid and its ability of antioxidant under elevated ultraviolet-B radiation (280–320 nm) was studied. The results showed flavonoid content in soybean seedlings with UV-B treatment during the stress and convalescent period was increased initially and then decreased, compared with control. Membrane permeability and MDA contents increase at first (first to fifth day) and then decrease (6th–11th day). A similar change of flavonoid content and clearance of flavonoid scavenging \({\text{O}}_2^ - \) and ·OH in soybean seedlings occurred. Flavonoid content and ability of flavonoid scavenging \({\text{O}}_2^ - \) and ·OH in soybean seedlings with La(III) + UV-B treatment were higher than those of UV-B treatment. Meanwhile, membrane permeability and MDA contents in soybean seedlings were lower than those of UV-B treatment. Compared with control, phenylalanine content in soybean seedlings with UV-B treatment is depressed, phenylalanine content in soybean seedlings with La(III) treatment was enhanced. However, phenylalanine content in La(III) + UV-B treatment is not decreased but slightly increased, compared with UV-B treatment. It suggested that the regulative effect of La(III) of the optimum concentration on flavonoid improved the metabolism of ROS, diminished the concentration of MDA and maintained normal plasma membrane permeability, and that its protective effect against low UV-B radiation is superior to that of high UV-B radiation. The defensive effect of La(III) on soybean seedlings under UV-B stress is carried out on the layer of defense system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

La(III):

lanthanum(III)

MDA:

malonaldehyde

\({\text{O}}_2^ - \) :

Superoxide radical

·OH:

hydroxyl radical

UV-B:

ultraviolet-B radiation

RMP:

relative membrane permeability

SOD:

superoxide dismutase

PAL:

phenylalanine ammonia-lyase

Phe:

phenylalanine

ROS:

reactive oxygen species

References

  1. Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504

    Article  PubMed  CAS  Google Scholar 

  2. Frohnmeyer H, Staiger D (2003) Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol 133:1420–1428

    Article  PubMed  CAS  Google Scholar 

  3. Pourcel L, Routaboul JM, Cheynier V et al (2007) Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant Sci 12:29–36

    Article  PubMed  CAS  Google Scholar 

  4. Huang XH, Zhou Q, Zhang GS (2005) Advances on rare earth application in pollution ecology. J Rare Earths 23:5–11

    Google Scholar 

  5. Caldwell MM (1977) Restarch in photobiology. Plenum, New York, pp 13–17

    Google Scholar 

  6. Arvouet-Grand A, Vennat B, Pourrat A et al (1994) Standardisation d’un extrait de propolis et identification des principaux constituants. J Pharm Belg 49:462–468

    PubMed  CAS  Google Scholar 

  7. Dhindsa RS, Plumbdhindsa P, Thorpe TA (1981) Leaf senescence—correlated with increased levels of membrane-permeability and lipid-peroxidation, and decreased levels of superoxide-dismutase and catalase. J Exp Bot 32:93–101

    Article  CAS  Google Scholar 

  8. Janero DR (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indexes of lipid-peroxidation and peroxidative tissue-injury. Free Radical Biol Med 9:515–540

    Article  CAS  Google Scholar 

  9. Yu HH, Seo SJ, Kim YH et al (2006) Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OC1 auditory cells through scavenging free radicals. J Ethnopharmacol 107:383–388

    Article  PubMed  Google Scholar 

  10. Halliwell B, Gutteridge JMC, Aruoma OI (1987) The deoxyribose method—a simple test-tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 165:215–219

    Article  PubMed  CAS  Google Scholar 

  11. Osborne DR, Voogt P (1978) The analysis of nutrients in foods. Academic, New York (USA), pp 239–240

    Google Scholar 

  12. Zhao D, Reddy KR, Kakani VG et al (2003) Growth and physiological responses of cotton (Gossypium hirsutum L.) to elevated carbon dioxide and ultraviolet-B radiation under controlled environmental conditions. Plant Cell Environ 26:771–782

    Article  Google Scholar 

  13. Liang CJ, Huang XH, Zhou Q (2005) Effect of rare earths on plant under supplementary ultraviolet-B radiation: I effect of cerium on growth and photosynthesis in rape seedlings exposed to supplementary ultraviolet-B radiation. J Rare Earth 23:569–575

    Google Scholar 

  14. Grotewold E (2006) The genetics and biochemistry of floral pigments. Annu Rev Plant Biol 57:761–780

    Article  PubMed  CAS  Google Scholar 

  15. Liang B, Zhang GS, Zhou Q (2006) Effect of Lanthanum on the content of flavonoids in soybean seedling under ultraviolet-B stress: I effect of Lanthanum on total flavonoids. J Agro-Environ Sci 25:584–586

    CAS  Google Scholar 

  16. Salin ML (1988) Toxic oxygen species and protective systems of the chloroplast. Physiol Plantarum 72:681–689

    Article  CAS  Google Scholar 

  17. Xie ZB, Zhu JG, Chu HY et al (2003) Effect of lanthanum on rice growth and physiological parameters with split-root nutrient solution culture. J Rare Earth 21:86–91

    Google Scholar 

  18. Peng Q, Peng Q, Zhou Q (2008) Effect of lanthanum on the flavonoids in the soybean seeding under Ultraviolet-B stress: II effect on content of flavonoids. J Agro-Environ Sci 27:462–466

    Google Scholar 

  19. Nitz GM, Grubmuller E, Schnitzler WH (2004) Differential flavonoid response to PAR and UV-B light in chive (Allium schoenoprasum L.). Acta Hort 659:825–830

    CAS  Google Scholar 

  20. Amic D, Davidovic-Amic D, Beslo D (2003) Structure-radical scavenging activity relationships of flavonoids. Croat Chem Acta 76:55–61

    CAS  Google Scholar 

  21. Zhang HY, Chen DZ (2000) Theoretical elucidation on activity differences of ten flavonoid antioxidants. Acta Bioch Bioph Sin 32:317–321

    CAS  Google Scholar 

  22. Heim KE, Tagliaferro AR, Bobilya DJ (2002) Flavonoid antioxidants: chemistry, metabolism and structure—activity relationships. J Nutr Biochem 13:572–584

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by Project supported by the National Natural Science Foundation of China (30570323) and the Foundation of State Planning Committee (IFZ20051210).

Author information

Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Qing Zhou

  2. School of Environment and Civil Engineering, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Qi Peng & Qing Zhou

Authors
  1. Qi Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qing Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qing Zhou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peng, Q., Zhou, Q. Antioxidant Capacity of Flavonoid in Soybean Seedlings under the Joint Actions of Rare Earth Element La(III) and Ultraviolet-B Stress. Biol Trace Elem Res 127, 69–80 (2009). https://doi.org/10.1007/s12011-008-8218-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-008-8218-4

Keywords

Search

Navigation