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Effects of Ce3+ on Conformation and Activity of Superoxide Dismutase

Biological Trace Element Research volume 125, pages 170–178 (2008)Cite this article

Abstract

Ce3+ in various concentrations was added to superoxide dismutase (SOD) from rat eryhrocyte in vitro to gain insight into the mechanism of molecular interactions between Ce3+ and SOD. The results showed that the reaction between SOD and Ce3 was two order, which meant that the SOD activity was markedly accelerated by a low concentration of Ce3+ and inhibited by a high concentration of Ce3+. The spectroscopic assays suggested that the Ce3+ was determined to directly bind to SOD; the binding site of Ce3+ to SOD was 0.96, and the binding constants (K A) were 6.78 × 105 and 1.68 × 105L·mol−1; the binding Ce3+ entirely altered the secondary structure of SOD. It implied that the Ce3+ coordination created a new metal ion-active site form in SOD, thus leading to an enhancement in SOD activity.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 30470150, 20671067) and the Medical Development Foundation of Soochow University (grant no. EE120701).

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  1. Medical College of Soochow University, Suzhou, 215123, People’s Republic of China

    Jie Liu, Linglan Ma, Sitao Yin & Fashui Hong

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  1. Jie Liu
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  2. Linglan Ma
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  3. Sitao Yin
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  4. Fashui Hong
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Correspondence to Fashui Hong.

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Liu, J., Ma, L., Yin, S. et al. Effects of Ce3+ on Conformation and Activity of Superoxide Dismutase. Biol Trace Elem Res 125, 170–178 (2008). https://doi.org/10.1007/s12011-008-8165-0

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  • DOI: https://doi.org/10.1007/s12011-008-8165-0

Keywords

  • Cerium
  • Superoxide dismutase
  • Activity
  • Conformation