The effect of manganese (Mn) supplementation on bone mineral density (BMD) and bone metabolism parameters was determined in ovariectomized Sprague–Dawley rats. Rats were divided into four groups (OVX, OVX+Mn, sham, sham+Mn) and fed with different intake levels of manganese (adequate 0.001% Mn, supplementation 0.01% Mn) for 12 weeks. BMD of the lumbar vertebrae, femur, and tibia were significantly lowered in ovariectomized rats compared to the sham group. In addition, BMD of the lumbar vertebrae was significantly increased by Mn supplementation in the sham groups. Serum C-telopeptide cross-links of type I collagen (CTx), bone resorption biomarker, alkaline phosphatase (ALP), and bone formation biomarkers were not significantly different among the four groups. However, serum osteocalcin, a more sensitive bone formation biomarker, was significantly increased by Mn supplementation. To summarize, Mn supplementation resulted in increased BMD and bone formation. Based on our findings, more research is needed to better understand the effects of manganese supplementation on bone formation and resorption.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Sheng MH, Taper LJ, Veit H, Qian H, Ritchey SJ, Lau KH (2001) Dietary boron supplementation enhanced the action of estrogen, but not that of parathyroid hormone, to improve trabecular bone quality in ovariectomized rats. Biol Trace Element Res 82:109–123
Evans EM, Racette SB, Van Pelt RE, Peterson LR, Villareal DT (2007) Effects of soy protein isolate and moderate exercise on bone turnover and bone mineral density in postmenopausal women. Menopause 14:481–488
Hodges SJ, Pilkington M, Stamp TC et al (1991) Depressed levels of circulating menaquinones in patients with osteoporotic fractures of the spine and femoral neck. Bone 12:387–389
Relea P, Revilla M, Ripoll E, Arribas I, Villa LF, Rico H (1995) Zinc, biochemical markers of nutrition, and type I osteoporosis. Age Ageing 24:303–307
Dawson-Hughes B, Harris SS, Krall EA, Dallal GE (1997) Effect of calcium and vitamin D supplementation on bone density in men and women 65years of age or older. N Engl J Med 337:670–676
Munger RG, Cerhan JR, Chiu BCH (1999) Prospective study of protein intake and risk of hip fracture in postmenopausal women. Am J Clin Nutr 69:147–152
Nordin BEC (1988) Calcium in human biology. Springer, Heidelberg
Ministry of Health and Welfare (2006) Report on 2005 national health and nutrition examination survey—health behavior of adults. Ministry of Health and Welfare, Seoul, South Korea
Strause L, Saltman P, Smith KT, Bracker M, Andon MB (1994) Spinal bone loss in postmenopausal women supplemented with calcium and trace minerals. J Nutr 124:1060–1064
Underwood EJ (1981) Trace metals in human and animal health. J Hum Nutr 35:37–48
Lampugnani MG, Barnasconi S, Neri P, Lozzi L, Gavazzi I, Marchisio PC, Dejana E (1991) Role of manganese in MG-63 ostosarcoma cell attachment to fibrinogen and von Willebrand factor. Lab Invest 65:96–103
Rico H, Gómez-Raso N, Revilla M, Hernández ER, Seco C, Páez E, Crespo E (2000) Effects on bone loss of manganese alone or with copper supplement in ovariectomized rats. A morphometric and densitomeric study. Eur J Obstet Gynecol Reprod Biol 90:97–101
Lüthen F, Bulnheim U, Müller PD, Rychly J, Jesswein H, Nebe JG (2007) Influence of manganese ions on cellular behavior of human osteoblasts in vitro. Biomol Eng 24:531–536
Reeves PG, Nielsen FH, Fahey GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951
O’Loughlin PD, Morris HA (2003) Oophorectomy acutely increases calcium excretion in adult rats. J Nutr 133:2277–2280
Kalu DN, Liu CC, Hardin RR, Hollis BW (1989) The aged rat model of ovarian hormone deficiency bone loss. Endocrinology 124:7–16
Yamaura M, Nakamura T, Tsurukami H, Hijioka A, Narusawa K, Ohnishi H, Ohta T, Hosoda K (1996) Local bone turnover in the metaphysis of the proximal tibia and the lumbar vertebra during the early periods after ovariectomy in rats. Calcif Tissue Int 58:52–59
Leach RM, Harris DE (1997) Manganese. In: O’Dell BL, Sunde RA (eds) Handbook of nutritionally essential mineral elements. Marcel Dekker, New York, pp 335–355
Okano T (1996) Effects of essential trace elements on bone turnover—in relation to the osteoporosis. Nippon Rinsho 54:148–154
Ries WL, Key LL, Rodriguiz RM (1992) Nitroblue tetrazolium reduction and bone resorption by osteoclasts in vitro inhibited by a manganese-based superoxide dismutase mimic. J Bone Miner Res 7:931–939
Shingu M, Takahashi S, Ito M, Hamamatu N, Suenaga Y, Ichibangase Y, Nobunaga M (1994) Anti-inflammatory effects of recombinant human manganese superoxide dismutase on adjuvant arthritis in rats. Rheumatol Int 14:77–81
About this article
Cite this article
Bae, YJ., Kim, MH. Manganese Supplementation Improves Mineral Density of the Spine and Femur and Serum Osteocalcin in Rats. Biol Trace Elem Res 124, 28–34 (2008). https://doi.org/10.1007/s12011-008-8119-6
- Bone mineral density