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The Influence of Methionine, Selenomethionine, and Vitamin E on Liver Metabolic Pathways and Steatosis in High-Cholesterol Fed Rabbits

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Abstract

Significant disorders of liver metabolic pathways enzymes after high-cholesterol diet could give information on liver steatosis development. This process could probably also be inhibited by some compounds, as examined in rabbits. Forty-two male rabbits were served a high-cholesterol diet (2 g%) (0.67 g/kg b.m./24 h) with addition of d,l-methionine (70 mg/kg b.m./24 h) or seleno-d,l-methionine (12.5 μg/kg b.m./24 h) or α-tocopherol (10 mg/kg b.m./24 h) for 3 months to compare the protection effect of used compounds on liver metabolism and steatosis. At the beginning and every month, blood was taken. After the experiment was completed, livers were dissected for histological examinations. The concentration of total cholesterol (t-CH), triacylglycerol (TG), and the activities of aldolase (ALD), sorbitol dehydrogenase (SDH), glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were determined. Plasma t-CH and TG concentrations were significantly higher in all experimental groups vs control group. Blood serum AST and ALT activities did not undergo change but there were observed not significant increase in the CH group vs control group. Activities of SDH, GLDH, and LDH increased in blood serum and decreased in the liver in all experimental groups. Activities of LDH and SDH increased in the liver in the CH+Met group vs CH group. ALD activity decreased in the liver only in the CH and CH+Se groups. This data support a lipotoxic model of cholesterol-mediated hepatic steatosis. Prolonged administration of high-cholesterol diet not only disturbs the structure of cell membranes, which is expressed by decreased activity of enzymes in the liver and the migration of those enzymes to plasma but as well leads to steatosis of the liver, which has been confirmed by histological examinations. The applied compounds appear to have a varying influence upon the activity of enzymes determined in serum and liver. Obtained results showed a beneficial influence of methionine and vitamin E supplementation on liver steatosis development.

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References

  1. Wójcicki J, Barcew-Wiszniowska B, Krasowska B, Różewicka L, Samochowiec L (1991) Hepatoprotective effect of selenium and vitamin E in rabbits fed a high-fat diet. Folia Biol 39:37–47

    Google Scholar 

  2. Kartal Ozer N, Negis Y, Aytan N (2003) Molecular mechanism of cholesterol or homocysteine effect in the development of atherosclerosis: role of vitamin E. Biofactors 19:63–70

    PubMed  Google Scholar 

  3. Unger RH (2002) Lipotoxic diseases. Annu Rev Med 53:319–336

    Article  PubMed  CAS  Google Scholar 

  4. Mischoulon D, Fava M (2002) Role of S-adenosyl-l-methionine in the treatment of depression: a review of the evidence. Am J Clin Nutr 76:1158–1161

    Google Scholar 

  5. Slyshenkov VS, Shevalye AA, Liopo AV, Wojtczak L (2002) Protective role of l-methionine against free radical damage of rat brain synaptosomes. Acta Biochim Pol 49(4):907–916

    PubMed  CAS  Google Scholar 

  6. Mukhopadhyay-Sardar S, Rana MP, Chatterjee M (2000) Antioxidant associated chemoprevention by selenomethionine in murine tumor model. Mol Cell Biochem 206(1–2):17–25

    Article  PubMed  CAS  Google Scholar 

  7. Schrauzer GN (2000) Selenomethionine: a review of its nutritional significance, metabolism and toxicity. J Nutr 130:1653–1656

    PubMed  CAS  Google Scholar 

  8. Komaratat P, Chupukcharoen N, Wilairat P (1985) Effect of vitamin E on cholesterol plasma lipoprotein distribution and metabolism in rabbit. International Journal of Vitamin Research 20:167–171

    Google Scholar 

  9. Lowry OH, Rosenbrough NJ, Farr AR, Randall RL (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  10. Krawczyński J (1972) Enzymologist diagnostic in practice medicine. PZWL, Warsaw, pp 85–106

    Google Scholar 

  11. Zawistowski S (1986) Histology technique and basics of histopathology. PZWL, Warsaw

    Google Scholar 

  12. Jolly SR, Kane WJ, Bailie MB, Abrams GD, Lucchesi BR (1984) Canine myocardial reperfusion injury: its reduction by the combined administration of superoxide dismutase and catalase. Circ Res 54:277–285

    PubMed  CAS  Google Scholar 

  13. Jeon SM, Bok SH, Jang MK, Kim YH, Nam KT, Jeong TS, Park YB, Choi MS (2002) Comparison of antioxidant effects of naringin and probucol in cholesterol-fed rabbits. Clin Chim Acta 317:181–190

    Article  PubMed  CAS  Google Scholar 

  14. Wissler RW (1992) Theories and new horizons in the pathogenesis of atherosclerosis and the mechanisms of clinical effects. Arch Pathol Lab Med 116:1281–1291

    PubMed  CAS  Google Scholar 

  15. Seifalian AM, Piasecki C, Agarwal A, Davidson BR (1999) The effect of graded steatosis on flow in the hepatic parenchymal microcirculation. Transplantation 68:780–784

    Article  PubMed  CAS  Google Scholar 

  16. Jayasooriya AP, Sakono M, Yukizaki C, Kawano M, Yamamoto K, Fukuda N (2000) Effects of Momordica charantia powder on serum glucose concentration and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets. J Ethnopharmacol 72:331–336

    Article  PubMed  CAS  Google Scholar 

  17. Simons K, Ikonen E (2000) How cells handle cholesterol. Science 290:1721–1726

    Article  PubMed  CAS  Google Scholar 

  18. Mathews CK, Van Holde KE, Ahern KG (2000) Lipid metabolism II: membrane lipids steroids, isoprenoids and eicosanoic. In: Biochemistry, 3rd edn. Benjamin Cummings, San Francisco, p 659

  19. Griendling KK, Alexander RW (1997) Oxidative stress and cardiovascular disease. Circulation 96:3264–3265

    PubMed  CAS  Google Scholar 

  20. Young VR (1981) Selenium: a case for its essentiality in man. N Engl J Med 304:1228–1230

    Article  PubMed  CAS  Google Scholar 

  21. Bedwal RS, Nair N, Sharma MP, Mathur RS (1993) Selenium—its biological perspectives. Med Hypotheses 41:150–159

    Article  PubMed  CAS  Google Scholar 

  22. Zachara BA, Trafikowska U, Adamowicz A, Nartowicz E, Manitius J (2001) Selenium, glutathione peroxidases, and some other antioxidant parameters in blood of patients with chronic renal failure. J Trace Elem Med Biol 15:161–166

    Article  PubMed  CAS  Google Scholar 

  23. Zachara BA, Adamowicz A, Trafikowska U, Trafikowska A, Manitius J, Nartowicz E (2001) Selenium and glutathione levels, and glutathione peroxidase activities in blood components of uremic patients on hemodialysis supplemented with selenium and treated with erythropoietin. J Trace Elem Med Biol 15:201–208

    Article  PubMed  CAS  Google Scholar 

  24. Zachara BA, Dobrzyński W, Trafikowska U, Szymański W (2001) Blood selenium and glutathione peroxidases in miscarriage. Br J Obstet Gynaecol 108:244–247

    Article  CAS  Google Scholar 

  25. Birkner E, Kasperczyk S, Zalejska-Fiolka J, Kasperczyk A, Birkner B (2006) Influence of selenomethionine and vitamin E on the antioxidative system in animals with experimentally induced hypercholesterolemia. Biol Trace Elem Res 111:137–150

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry in Zabrze, Medical University of Silesia, Katowice, Poland

    Ewa Birkner, Jolanta Zalejska-Fiolka, Aleksandra Kasperczyk, Sławomir Kasperczyk, Ewa Grucka-Mamczar & Katarzyna Birkner

  2. Department of Pathology in Sosnowiec, Medical University of Silesia, Katowice, Poland

    Barbara Stawiarska-Pięta

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  1. Ewa Birkner
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  2. Jolanta Zalejska-Fiolka
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  3. Aleksandra Kasperczyk
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  4. Sławomir Kasperczyk
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  6. Barbara Stawiarska-Pięta
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  7. Katarzyna Birkner
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Correspondence to Jolanta Zalejska-Fiolka.

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Birkner, E., Zalejska-Fiolka, J., Kasperczyk, A. et al. The Influence of Methionine, Selenomethionine, and Vitamin E on Liver Metabolic Pathways and Steatosis in High-Cholesterol Fed Rabbits. Biol Trace Elem Res 120, 179–194 (2007). https://doi.org/10.1007/s12011-007-0070-4

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  • DOI: https://doi.org/10.1007/s12011-007-0070-4

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