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Microcalorimetric Investigation on Metabolic Activity and Effects of La (III) in Mitochondria Isolated from Indica Rice 9311

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Abstract

Thermogenic metabolic curves were determined by the ampoule method at 303 K using a TAM air isothermal microcalorimeter in mitochondria isolated from rice 9311 (Oryza sativa L). From the thermogenic curves the activity recovery rate constant k and the maximum heat power P m were obtained. Both were positively correlated to the protein content of rice mitochondria. The corresponding correlation coefficients were 0.9959 and 0.9950, respectively, indicating that the in vitro metabolic activity of mitochondria can be reliably expressed by these parameters. Addition of La (III) ions in concentrations ranging from 0 to 130 μg/mL resulted in significantly higher k and P m values. Concentrations from 140 to 180 μg/mL had the opposite effect. These results are consistent with previous reports on the effects of rare earth elements on plant growth. We propose that the lanthanum-induced change of mitochondrial metabolic activity is a possible mechanism by which La (III) ions influence indica rice 9311 growth.

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Acknowledgments

We gratefully acknowledge financial support of project supported by the National Natural Science Foundation of China (NO: 30570015, 20621502); Natural Science Foundation of Hubei Province (2005ABC002); and Science Research Foundation of Chinese Ministry of Education (NO: [2006]8IRT0543), and the Doctoral Program from the State Education Ministry of China, and the Research Program of Hubei Provincial Department of Education, China (No. 2004X048).

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Authors and Affiliations

  1. Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Jie Dai, Ye-Zhong Zhang & Yi Liu

  2. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, 434025, People’s Republic of China

    Jie Dai, Ye-Zhong Zhang & Yi Liu

  3. State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yi Liu

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  1. Jie Dai
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  2. Ye-Zhong Zhang
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  3. Yi Liu
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Correspondence to Yi Liu.

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Dai, J., Zhang, YZ. & Liu, Y. Microcalorimetric Investigation on Metabolic Activity and Effects of La (III) in Mitochondria Isolated from Indica Rice 9311. Biol Trace Elem Res 121, 60–68 (2008). https://doi.org/10.1007/s12011-007-0062-4

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  • DOI: https://doi.org/10.1007/s12011-007-0062-4

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