Abstract
The toxic effect of Pb2+ has been studied in eukaryotic cells by using Tetrahymena as a target. The maximum power (P m) and the growth rate constant (k) were determined, which showed that values of P m and k were linked to the concentration (C) of Pb2+. The addition of Pb2+ caused a decrease of the maximum heat production and growth rate constant, indicating that Tetrahymena growth was inhibited in the presence of Pb2+, and Pb2+ took part in the metabolism of cells. From micrographs, morphological changes of Tetrahymena were observed with addition of Pb2+, indicating that the toxic effect of Pb2+ derived from destroying the membrane of surface of Tetrahymena. According to the thermogenic curves and photos of Tetrahymena under different conditions, it is clear that metabolic mechanism of Halobacterium halobium R1 growth has been changed with the addition of Pb2+.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Shen YF (1999) Protozoology. Science Press, Beijing, pp. 456–496
Csaba G, Kovács P, Pállinger É (2006) Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena. Cell Bio Int 30(4):345–348
McPartland JM, Matias I, Di Marzo V, Glass M (2006) Evolutionary origins of the endocannabinoid system. Gene 370(29):64–74
Novotný Č, Dias N, Kapanen A, Malachová K, Vándrovcová M (2006) Merja Itävaara and Nelson Lima. Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. Chemosphere 63(9):1436–1442
Saygideger S, Dogan M (2005) lnfluence of pH on lead uptake, chlorophyll and nitrogen content of Mentha aquatica L. and Nasturtium officinale R.Br. J Environ Biol 26(4):753–759
Chetty CS, Vemuri, MC, Campbell, KSC (2005) Lead induced cell death of human neuroblastoma cells involves GSH deprivation. Cell Mol Biol Lett 10(3):413–423
Caiqin Q, Bo Z, Lintao Z, Zhonghai Z, Yi L, Yumin D, Ling X (2004) The physicochemical properties and antitumor activity of cellulase-treated chitosan. Food Chem 84(1):107–115
Peng L, Yi L, Zhixiong X, Jie G, Anxin H, Ping S, Songsheng Q (2005) Microcalorimetric studies of the action of Er3+ on Halobacterium halobium R1 growth. Biol Trace Elem Res 104(3):275–284
Jun Y, Yi L, Peng L, Zhenting G, Ming S, Songsheng Q, Ziniu Y, Yunfen S (2003) Microcalorimetric investigations of influence of Mn(II) on the growth of Tetrahymena shanghaiensis S1 99. Biol Trace Elem Res 92(1):71–82
Peng L, Yi L, Zhixiong X, Ping S, Songsheng Q (2003) Microcalorimetric studies of the toxic action of La3+ on Halobacterium halobium R1 growth. Chin J Chem 21(6):693–697
Acknowledgement
The project was supported by State Key Laboratory of Advanced Technology for Materials Synthesis and Processing (Wuhan University of Technology).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Peng, L., Weiying, Z., Xi, L. et al. Microcalorimetric Study on the Toxic Effect of Pb2+ to Tetrahymena . Biol Trace Elem Res 119, 175–182 (2007). https://doi.org/10.1007/s12011-007-0055-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-007-0055-3