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Bioenergetic Investigation of Action of Lithium to Tetrahymena thermophila bF5 by Microcalorimetry

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Abstract

Microcalorimetry was employed to investigate the action of Li(I) to aquatic ecosystem from the point view of bioenergetics. Tetrahymena thermophila BF5 was chosen as the model organism. The power-time curves of T. thermophila BF5 growth metabolism in the absence and presence of Li(I) were obtained. The corresponding thermokinetic parameters were derived. The generation time was calculated as 592.3 min, which was consistent with the biomass values. Low concentration of Li(I) (1–20 mmol l−1) stimulated the growth of T. thermophila BF5, whereas the inhibition effect was observed in high concentration (30–100 mmol l−1). The value of IC50 was 52.8 mmol l−1. In the concentration range of 30–100 mmol l−1, the growth rate constants (k) and the maximum heat out power (P max) decrease with the concentration of Li(I), whereas the heat output (Q) increases slightly compared to the control. Other than the classic mechanism of inositol-phosphate cycle, the involvement of mitochondria mechanism was discussed and suggested.

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Acknowledgment

This work was financially supported by the National Natural Science Foundation of China (No.30570015, 20472066 and 20373051), Science Fund for Creative Research Group (No.20621502 NSFC), Science Research Foundation of Chinese Ministry of Education (NO: [2006]8IRT0543), and Natural Science Foundation of Hubei Province (No.2005ABC02).

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Authors and Affiliations

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Hui-Rong Li, Yi Liu, Cai-Qin Qin & Qiang-Guo Li

  2. College of Chemistry and Environmental Engineering, Yangtze University, JingZhou, Hubei, 434025, China

    Yi Liu

  3. State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yi Liu

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  1. Hui-Rong Li
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  2. Yi Liu
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  4. Qiang-Guo Li
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Correspondence to Yi Liu.

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Li, HR., Liu, Y., Qin, CQ. et al. Bioenergetic Investigation of Action of Lithium to Tetrahymena thermophila bF5 by Microcalorimetry. Biol Trace Elem Res 119, 60–67 (2007). https://doi.org/10.1007/s12011-007-0040-x

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  • DOI: https://doi.org/10.1007/s12011-007-0040-x

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