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Microcalorimetric Studies of the Biological Effect of Holmium (III) on Halobacterium halobium R1 Growth

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Abstract

The biological effect of Ho3+ on Halobacterium halobium R1 growth was analyzed by a microcalorimetric technique. By means of LKB-2277 Bioactivity Monitor, ampoule method at 37°C, we obtained the thermogenic curves of H. halobium R1 growth. To analyze the results, the maximum power (P m) and the growth rate constants (k) were determined, which show that values of P m and k are linked to the concentration of Ho3+. In all, the addition of Ho3+ causes a decrease of the maximum heat production and growth rate constants. For comparison, we observed the shapes of H. halobium R1 cell by means of transmission electron microscope (TEM). According to the thermogenic curves and TEM photos of H. halobium R1 under different conditions, it is clear that metabolic mechanism of H. halobium R1 growth has been changed with the addition of Ho3+.

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Acknowledgment

We gratefully acknowledge the financial support of National Natural Science Foundation of China (30570015, 20373051), “973” Project (2004CB719603), Science Fund for Creative Research Group (No.20621502 NSFC), Science Research Foundation of Chinese Ministry of Education (NO: [2006]8IRT0543), and Natural Science Foundation of Hubei Province (No.2005ABC02).

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Authors and Affiliations

  1. Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Liu Peng & Liu Yi

  2. Department of Applied Chemistry, School of Sciences, Wuhan University of Technology, Wuhan, 430070, People’s Republic of China

    Liu Peng & Li Xi

  3. College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Shen Ping

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  1. Liu Peng
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  2. Liu Yi
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  3. Li Xi
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  4. Shen Ping
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Correspondence to Liu Yi.

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Peng, L., Yi, L., Xi, L. et al. Microcalorimetric Studies of the Biological Effect of Holmium (III) on Halobacterium halobium R1 Growth. Biol Trace Elem Res 121, 80–86 (2008). https://doi.org/10.1007/s12011-007-0039-3

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  • DOI: https://doi.org/10.1007/s12011-007-0039-3

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