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Cloning, Purification, and Biophysical Characterization of FemB Protein from Methicillin-Resistant Staphylococcus aureus and Inhibitors Screening

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Abstract

Methicillin-resistant Staphylococcus aureus has emerged as a leading cause of nosocomial, community acquired infections worldwide. Earlier investigations revealed that mecA-encoded FEM proteins play a role in antimicrobial resistance by developing unique peptidoglycan cross-linking which helps in the formation of protective cell membrane. In view to this, present study focused on expression, purification FEM proteins, and FemB biophysical characterization with the aid of in silico and in vitro approaches. Furthermore, we carried out biological screening assays and identified the novel potent 1,2,3-triazole conjugated 1,3,4-oxadiazole hybrid molecule which could inhibit the MRSA than the proven oxacillin.

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Data Availability

All datasets generated for this study are included in the article. The data used to support the findings of this study are available from the corresponding author upon request.

Abbreviations

FDA:

U.S. Food and Drug Administration

PDB:

Protein Data Bank

MRSA:

Methicillin-Resistant Staphylococcus aureus

FEM:

Factors Essential for Methicillin-resistance

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Acknowledgements

We would also like to express our gratitude to the Centre for Microbial Fermentation Technology (CMFT)-Microbiology, Rashtriya Uchchatar Shiksha Abhiyan (RUSA 2.0), Central Facilities for Research and Development-Osmania University (CFRD-OU) and Centre for Cellular & Molecular Biology (CCMB), Hyderabad, for providing the necessary facilities and infrastructure.

Funding

The authors express their sincere thanks to DST-SERB-ECR (Grant file no: ECR/2017/003381) for financial support.

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Authors and Affiliations

  1. Molecular Medicine Lab, Dept. of Genetics & Biotechnology, Osmania University, Hyderabad, Telangana, 500007, India

    Anjini Gayatri Akkiraju & Someswar Rao Sagurthi

  2. Department of Chemistry, Nizam College, Osmania University, Hyderabad, Telangana, 500001, India

    Krishnam Raju Atcha

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Contributions

SRS conceived the idea and provided critical inputs to the concept and also supervised the progress of the project. AGA performed the biological experiments and molecular docking. KRA generated the data for ligand synthesis. All the authors are involved in data interpretation, manuscript writing, and approved the manuscript for publication.

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Correspondence to Someswar Rao Sagurthi.

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Akkiraju, A.G., Atcha, K.R. & Sagurthi, S.R. Cloning, Purification, and Biophysical Characterization of FemB Protein from Methicillin-Resistant Staphylococcus aureus and Inhibitors Screening. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04780-8

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