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Influence of Borassus flabellifer Endocarps Hydrolysate on Fungal Biomass and Fatty Acids Production by the Marine Fungus Aspergillus sp.

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Abstract

Polyunsaturated Fatty Acids (PUFAs) are important nutrients for human health. We aimed to evaluate the efficiency of marine water fungus Aspergillus sp. (Accession no: MZ505709) for lipid biosynthesis. The Yeast Extract Glucose (YEG) medium was supplemented with different concentration of Borassus flabellifer Endocarps Hydrolysate (BFEH; 1–5%) to evaluate the fungal biomass and its lipid accumulation. The combination of glucose and BFEH as carbon source increased the fresh weight (25.43 ± 0.33 g/L), dry weight (21.39 ± 0.77 g/L) and lipid yield (3.14 ± 0.09 g/L) of fungal biomass. The lipid content of dried fungal biomass has shown 91.08 ± 5.07 mg cod liver oil equivalents/g and 125.98 ± 5.96 mg groundnut oil equivalents/g biomass. GC-MS and NMR spectrometry analysis revealed the compounds involved in fatty acid metabolism and lipid signaling pathways along with the presence of linolenic acid. Interestingly, fungus grown in BFEH enriched medium has recorded the maximum amount of lipids with major fatty acid derivatives. Increase in the growth rate of Artemia franciscana was observed, when the extracted fungal lipid was supplemented as a food supplement. Therefore, this study suggests that marine fungal lipid may serve as potential natural compound as nutraceuticals and aquafeeds.

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Data Availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are thankful to the (UGC-SAP), New Delhi, and DST-FIST II Programme, Department of Botany for providing the financial support to carry out the research work. The authors are thankful to National Higher Education Mission (RUSA 2.0- BEICH), New Delhi, to carry out the research work fruitfully.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Botany, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    Nancy Mary Thomas, Vasanthkumar Rajkumar & Gurusaravanan Packiaraj

  2. Department of Biotechnology, Sona College of Arts and Science, Salem, 636 005, Tamil Nadu, India

    Vinoth Sathasivam

  3. Department of Zoology, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    Muralisankar Thirunavukarasu & Gayathri Velusamy

  4. Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    Arun Muthukrishnan

  5. Department of Biochemistry, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    Saradhadevi Muthukrishnan

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Work design, conceptualization and writing the manuscript was performed by Gurusaravanan Packiaraj, Nancy Mary Thomas and Vasanthkumar Rajkumar. Equal contribution to the work was given by Muralisankar Thirunavukarasu, Saradhadevi Muthukrishnan and Gayathri Velusamy. Data validation and analysis were performed by Vinoth Sathasivam and Arun Muthukrishnan. All authors read and approved the final manuscript.

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Correspondence to Gurusaravanan Packiaraj.

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Thomas, N.M., Sathasivam, V., Thirunavukarasu, M. et al. Influence of Borassus flabellifer Endocarps Hydrolysate on Fungal Biomass and Fatty Acids Production by the Marine Fungus Aspergillus sp.. Appl Biochem Biotechnol 196, 923–948 (2024). https://doi.org/10.1007/s12010-023-04588-6

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