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Complete Genome Sequencing and Bacteriocin Functional Characterization of Pediococcus ethanolidurans CP201 from Daqu

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Abstract

This study aims to sequence the whole genome of Pediococcus ethanolidurans CP201 isolated from Daqu and determine the anti-corrosion ability of bacteriocins on chicken breast. The whole genome sequence information of P. ethanolidurans CP201 was analyzed, and its gene structure and function were explored. It was found that gene1164 had annotations in the NR, Pfam, and Swiss-Prot databases, and was related to bacteriocins. The exogenous expression of the bacteriocin gene Pediocin PE-201 was analyzed based on the pET-21b vector and the host BL21, and the corresponding bacteriocin was successfully expressed under the induction of IPTG. After purification by NI–NTA column, enterokinase treatment, membrane dialysis concentration treatment, and SDS-PAGE electrophoresis, the molecular weight was about 6.5 kDa and the purity was above 90%. By applying different concentrations of bacteriocin to chicken breast with different levels of contamination, the control of pathogenic bacteria, the ordinary contamination level (OC) group, and the high contamination level (MC) group could be completely achieved with 25 mg/L bacteriocin. In conclusion, the bacteriocin produced by the newly isolated CP201 can be applied to the preservation of meat products to prevent the risk of food-borne diseases.

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The author states that the data and other documents supporting the results of this research can be found in the article. In addition, the sequence data reported in this paper has been stored in NCBI SRA database (PRJNA884871).

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Funding

This work was supported by the National Natural Science Foundation of China (Project No. 30660005), the Sichuan Tujiu Liquor Co., Ltd (Project No. 222305), the Protect of Chengdu Shuzhiyuan Liquor Co., Ltd (Project No. 202306), the Protect of Chengdu Technology Innovation (Project No. 2022-YF05-00136-SN), and the Project of Sichuan Institute of International Science and Technology Cooperation (Australia and New Zealand) (Project No. AXYJ2022 -005).

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Authors and Affiliations

  1. College of Food & Bioengineering, Xihua University, Chengdu, 610039, China

    Tongwei Guan, Liuzhu Long, Ying Liu, Lei Tian & Zhong Peng

  2. Sichuan Provincial Key Laboratory of Food Microbiology, Chengdu, 610039, China

    Tongwei Guan

  3. Sichuan Tujiu Liquor Co., Ltd., Nanchong, 637000, China

    Zongjun He

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  1. Tongwei Guan
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Contributions

LZL: writing—original draft preparation; YL: methodology; ZP: writing—review and editing; LT: data curation, software, validation; ZJH: data curation; TWG: funding acquisition, writing—reviewing and editing. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Tongwei Guan.

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Guan, T., Long, L., Liu, Y. et al. Complete Genome Sequencing and Bacteriocin Functional Characterization of Pediococcus ethanolidurans CP201 from Daqu. Appl Biochem Biotechnol 195, 4728–4743 (2023). https://doi.org/10.1007/s12010-023-04575-x

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