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Wound Healing Potential of Couroupita guianensis Aubl. Fruit Pulp Investigated on Excision Wound Model

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Abstract

Wound care management aims at stimulating and improving healing process without scar formation. Although various plants have been reported to possess wound healing properties in tribal and folklore medicines, there is a lack of scientific data to validate the claim. In this aspect, it becomes inevitable to prove the efficacy of naturally derived products at pharmacological levels. Couroupita guianensis as a whole plant has been reported to exhibit wound healing activity. The leaves and fruit of this plant have been utilized in folkloric medicine to cure skin diseases and infections for many years. However, to the best of our knowledge, no scientific studies have been conducted to verify the wound healing properties of C. guianensis fruit pulp. Therefore, the present study seeks to investigate the wound healing potential of C. guianensis fruit pulp using an excision wound model in Wistar albino male rats. This study indicated that the ointment prepared from crude ethanolic extract of C. guianensis fruit pulp facilitated wound contraction that were evidenced by a greater reduction in the wound area and epithelialization period and increased hydroxyproline content. The experimental groups treated with low and mid dose of C. guianensis ethanol extract (CGEE) ointments had shown a wound closure of 80.27% and 89.11% respectively within 15 days, which is comparable to the standard betadine ointment which showed 91.44% healing in the treated groups. Further, the extract influenced the expression of genes VEGF and TGF-β on post wounding days that clearly explained the strong correlation between these genes and wound healing in the experimental rats. The animals treated with 10% CGEE ointment showed a significant upregulation of both VEGF and TGF-β as compared with other test and standard groups. These findings provide credence to the conventional application of this plant in the healing of wounds and other dermatological conditions, and may represent a therapeutic strategy for the treatment of wounds.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code Availability

Not applicable.

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Authors and Affiliations

  1. PG & Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai, India

    L. Anna Sheba & V Anuradha

  2. Department of Biochemistry, Soka Ikeda College of Arts and Science for Women, Chennai, India

    L. Anna Sheba

  3. PG & Research Department of Biotechnology, Mohamed Sathak College of Arts and Science, Chennai, India

    M. Syed Ali & N Yogananth

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  1. L. Anna Sheba
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Contributions

Anna Sheba L.: conception, strategy, formal analysis and research, writing—first draft preparation, composing, reviewing, and rewriting the manuscript. Anuradha V.: review and editing the manuscript. Syed Ali M.: contributed statistical tools and analyzed the data. Yogananth N.: contributed statistical tools and analyzed the data.

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Correspondence to V Anuradha.

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The animal experiment protocol was approved by the Institute Animal Ethics Committee (IAEC no: LVII/02/321/PO/Re/S/04/CPCSEA).

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Sheba, L.A., Anuradha, V., Ali, M.S. et al. Wound Healing Potential of Couroupita guianensis Aubl. Fruit Pulp Investigated on Excision Wound Model. Appl Biochem Biotechnol 195, 6516–6536 (2023). https://doi.org/10.1007/s12010-023-04400-5

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