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Effects of the Polyphenols Delphinidin and Rosmarinic Acid on the Inducible Intra-cellular Aggregation of Alpha-Synuclein in Model Neuron Cells

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Abstract

Intracellular aggregation of α-synuclein is a major pathological feature of Parkinson’s disease. In this study, we show that the polyphenols delphinidin and rosmarinic acid suppress intracellular aggregation of α-synuclein in a mouse neuron cell model when added under oxidative stress conditions. To enhance the detection threshold of this preventive effect of the two polyphenols, we generated a new strain of “aggregation prone model cells” that tended to show prominent α-synuclein aggregation even under normal conditions. Using this new highly sensitive cell line, we demonstrate that addition of delphinidin to model cell cultures effectively suppresses the formation of intracellular α-synuclein aggregates. Flow cytometric analysis shows that adding delphinidin decreases the fraction of “dying cells,” cells that were alive but in a damaged state. Our findings suggest the possibility of using polyphenols to prevent and treat the symptoms correlated with the onset of Parkinson’s disease. Additionally, our aggregation-prone cell model may be used in future studies to probe numerous neurodegenerative diseases with high sensitivity.

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Data Availability

All data are presented in the article. All of the data that were used to produce the figures in the present study are available upon request from Y. K., Tottori University.

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Funding

This research was partially supported by financial aid from WAKASA SEIKATSU Co., Ltd., Japan.

Author information

Authors and Affiliations

  1. Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, 680-8552, Japan

    Hanae Yamamoto, Kunihiro Hongo, Tomohiro Mizobata & Yasushi Kawata

  2. Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Tottori, 680-8552, Japan

    Rio Matsumura, Mayuka Adachi, Kunihiro Hongo, Tomohiro Mizobata & Yasushi Kawata

  3. Department of Chemistry and Biotechnology, Faculty of Engineering, Tottori University, Tottori, 680-8552, Japan

    Miho Nakashima, Kunihiro Hongo, Tomohiro Mizobata & Yasushi Kawata

  4. Organization for promotion of Tenure Track, University of Miyazaki, Miyazaki, 889-2192, Japan

    Kenjirou Ogawa

  5. Center for Research on Green Sustainable Chemistry, Tottori University, Tottori, 680-8552, Japan

    Kunihiro Hongo, Tomohiro Mizobata & Yasushi Kawata

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  1. Hanae Yamamoto
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Contributions

Conceptualization, H.Y. and Y.K.; data curation, H.Y., R.M., M.N., and M.A.; formal analysis, H.Y. and Y.K.; funding acquisition, Y.K.; investigation, H.Y., K.O., K.H., T.M., and Y.K.; methodology, H.Y., R.M., and M.N.; supervision, H.Y., K.H., T.M., K.O., and Y.K.; validation, H.Y., T.M., and Y.K.; writing—original draft, H.Y. and Y.K.; writing—review and editing, H.Y., T.M., and Y.K.

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Correspondence to Yasushi Kawata.

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Yamamoto, H., Matsumura, R., Nakashima, M. et al. Effects of the Polyphenols Delphinidin and Rosmarinic Acid on the Inducible Intra-cellular Aggregation of Alpha-Synuclein in Model Neuron Cells. Appl Biochem Biotechnol 195, 4134–4147 (2023). https://doi.org/10.1007/s12010-023-04362-8

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