Abstract
The fabrication of multifunctional scaffolds has attracted much attention in biological fields. In this research, some novel composites of Cu(II) or Zn(II) metal–organic framework (M-MOF) and polycaprolactone (PCL), M-MOF@PCL, have been fabricated as multifunctional scaffolds for application in the tissue engineering (TE) field. The porous three-dimensional sponges were prepared by the salt leaching method. Then, the M-MOF@PCL composite sponges have been prepared by in situ synthesis of M-MOF in the presence of the as-obtained PCL sponge to gain a new compound with proper features for biological applications. Finally, curcumin was attached to the M-MOF@PCL as a bioactive compound that can act as a wound-healing agent, anti-oxidant, and anti-inflammatory. The presence of the M-MOF in final composites was investigated by different methods such as FTIR (Fourier-transform infrared), XRD (X-ray diffraction), SEM (scanning electron microscope), EDS (energy-dispersive X-ray spectroscopy), and TEM (transmission electron microscope). SEM images confirmed the porous structure of the as-obtained composites. According to the EDS and TEM images, M-MOFs were uniformly incorporated throughout the PCL sponges. The water sorption capacities of the blank PCL, Cu-MOF@PCL, and Zn-MOF@PCL were determined as 56%, 155%, and 119%, respectively. In vivo investigation on a third-degree burn model in adult male Wistar rats exhibited an accelerated wound healing for Cu-MOF@PCL compared to with Zn-MOF@PCL and the control group.
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References
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The authors received financial support (Grant No.: SCU.SC.98.20911) from the Shahid Chamran University of Ahvaz.
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Zeinab Ansari-Asl: writing original—draft, conceptualization, methodology, formal analysis, investigation, resources); Soghra Nikpour: conceptualization, methodology, formal analysis, investigation, resources, visualization; Tahereh Sedaghat: conceptualization, methodology, formal analysis, investigation; Elham Hoveizi: writing original—draft, conceptualization, methodology, formal analysis.
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Ansari-Asl, Z., Nikpour, S., Sedaghat, T. et al. Preparation, Characterization, and Wound Healing Assessment of Curcumin-Loaded M-MOF (M = Cu, Zn)@Polycaprolactone Nanocomposite Sponges. Appl Biochem Biotechnol 195, 4308–4320 (2023). https://doi.org/10.1007/s12010-023-04316-0
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DOI: https://doi.org/10.1007/s12010-023-04316-0