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Bladder Cancer Progression Is Suppressed Through the Heart and Neural Crest Derivatives Expressed 2-Antisense RNA 1/microRNA-93-5p/Defective in Cullin Neddylation 1 Domain Containing 3 Axis

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A Correction to this article was published on 13 April 2023

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Abstract

MicroRNAs (miRNAs) are critical in progression of bladder cancer (BCa). miRNA-93-5p is increased in cancers and is positively correlated with an unfavorable prognosis. But its effects on BCa remain rarely understood. This investigation aimed to dig out miRNA-93-5p affecting biological behaviors of BCa. In this research, mRNA and protein expression in cancer cells were assessed via quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Cell Counting Kit-8 (CCK-8), colony formation, scratch healing, and transwell assays were utilized to analyze cancer cell viability, colony-forming, migration, and invasion, respectively. Bioinformatics analysis predicted upstream regulatory genes and downstream target genes of miRNA-93-5p, with the targeting relationship being verified through a dual-luciferase assay. The BCa xenograft model in nude mice further investigated the effect of miRNA-93-5p and AND2-AS1 on tumor size and quality, and validated the relationship between HAND2-AS1/miRNA-93-5p/DCUN1D3. Our results displayed that miRNA-93-5p was increased in BCa cell lines. Knockdown miRNA-93-5p constrained BCa cell malignant phenotypes. HAND2-AS1 targeted miRNA-93-5p, thus restraining malignant progression of BCa cells. DCUN1D3 was found downstream of miRNA-93-5p. miRNA-93-5p modulated proliferation, migration, and invasion of BCa cells by targeting DCUN1D3. In vivo experiments disclosed that forced expression of lncRNA HAND2-AS1, and inhibited miRNA-93-5p regressed tumor growth. Meanwhile, the same as the results of cell experiments, the expression of miRNA-93-5p was downregulated, and DCUN1D3 expression was advanced in tumor tissues. To conclude, lncRNA HAND2-AS1 exerted anti-tumor effects and regulated BCa cell proliferation, invasion, and migration by targeting miRNA-93-5p/DCUN1D3.

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Data Availability

The data used to support the findings of this study are included within the article. The data and materials in the current study are available from the corresponding authors on reasonable request.

Materials Availability

The data used to support the findings of this study are included within the article. The data and materials in the current study are available from the corresponding authors on reasonable request.

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Acknowledgements

Not applicable.

Funding

The research was supported by high-level hospital foster grants from Fujian Provincial Hospital, Fujian Province, China (2020HSJJ13); Startup Fund for scientific research, Fujian Medical University (2019QH1175).

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Author notes

  1. Xiang Wu and Qingjiang Xu contributed equally to this work.

Authors and Affiliations

  1. Provincial Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China

    Xiang Wu, Qingjiang Xu, Tao Li, Yongbao Wei, Rong Zeng, Rongcheng Lin, Lina Xu, Liefu Ye & Zhihua Liu

  2. Department of Urology, Fujian Provincial Hospital, No. 516 Jinrong South Road, Cangshan District, Fuzhou, 350001, China

    Xiang Wu, Qingjiang Xu, Tao Li, Yongbao Wei, Rong Zeng, Rongcheng Lin & Liefu Ye

  3. Department of Urology, South Branch of Fujian Provincial Hospital, Fuzhou, 350001, China

    Lina Xu & Zhihua Liu

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  1. Xiang Wu
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Contributions

XW and QJ contributed to conception and design of the study. TL contributed to acquisition of data. YB contributed to data analysis. RZ drafted the manuscript. RC contributed to investigation. LN contributed to visualization. LF contributed to supervision and funding acquisition. ZH revised the article and gave final approval of the version to be published.

Corresponding authors

Correspondence to Liefu Ye or Zhihua Liu.

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Ethics Approval and Consent to Participate

This study was approved by the Medical Ethics Committee of Fujian Provincial Hospital (K2020-12–032). All participants signed written informed consent for research purposes and publication. Ethics Committee of Zhejiang Eyoung Pharmaceutical Research and Development Center Laboratory Animal Ethics Committee approved all animal surgeries in this study.

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Wu, X., Xu, Q., Li, T. et al. Bladder Cancer Progression Is Suppressed Through the Heart and Neural Crest Derivatives Expressed 2-Antisense RNA 1/microRNA-93-5p/Defective in Cullin Neddylation 1 Domain Containing 3 Axis. Appl Biochem Biotechnol 195, 4116–4133 (2023). https://doi.org/10.1007/s12010-022-04295-8

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