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lncRNA ENST00000585827 Contributes to the Progression of Endometrial Carcinoma via Regulating miR-424/E2F6/E2F7 Axis

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Abstract

Endometrial cancer (EC) ranks fourth among the most common gynecologic malignancies. Despite advances in medical technology, the pathogenesis is still unclear. Numerous reports have identified the involvement of lncRNA in the malignant progression of endometrial cancer. The aim of the study was to investigate the expression level of lncRNA ENST00000585827 (lncRNA E27) in endometrial cancer and the molecular mechanism that regulates the development of endometrial cancer. Combined with the results of the previous study, PCR analysis confirmed that lncRNA E27 was significantly upregulated in endometrial cancer cell lines. The results of CCK-8, wound healing assay, and transwell experiments showed that lncRNA E27 could significantly inhibit cell proliferation, migration, and invasion. Flow cytometry results confirmed that lncRNA E27 could promote apoptosis. Furthermore, based on bioinformatics predictions, dual-luciferase assay and RT-qPCR analysis confirmed that miR-424, as its downstream molecule, competitively regulates the expression of E2F6/E2F7. Rescue experiments further supported that lncRNA E27 inhibited proliferation, migration, invasion, and promoted apoptosis of endometrial cancer through miR-424/E2F6/E2F7 signaling axis. Conclusively, our findings revealed the role of lncRNA E27 in regulating the miR-424/E2F6/E2F7 signaling axis during EC progression, opening up new strategies for the treatment of endometrial cancer.

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All data in the article will be made available by the authors, without undue reservation.

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Funding

This project is supported by the National Natural Science Foundation of China (81860495).

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Authors and Affiliations

  1. Soochow University Medical College, Suzhou, China

    Derong Fang

  2. Zunyi Medical University, Zunyi, China

    Derong Fang, Qingfang Deng & Yun Wang

  3. Department of Obstetrics, Affiliated Hospital of Zunyi Medical University, Guizhou Province, Zunyi, 563000, China

    Derong Fang, Qiang Zhang & Quan Li

  4. Family Planning Department, Affiliated Hospital of Zunyi Medical University, Zunyi, China

    Maoling Mu

Authors
  1. Derong Fang
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  2. Qiang Zhang
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  3. Maoling Mu
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  4. Qingfang Deng
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Contributions

D. F.: writing, conceptualization, methodology, software.

Q. Z.: data curation, writing, original draft preparation.

M. M.: visualization, investigation.

Q. D.: supervision, methodology.

Y. W.: software, validation, data curation.

Q. L.: reviewing and editing.

Corresponding author

Correspondence to Quan Li.

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Ethical Approval

The animal experiments in the study were approved by the Animal Ethics Committee of the First Affiliated Hospital of Zunyi Medical University.

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The authors declare no competing interests.

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Fang, D., Zhang, Q., Mu, M. et al. lncRNA ENST00000585827 Contributes to the Progression of Endometrial Carcinoma via Regulating miR-424/E2F6/E2F7 Axis. Appl Biochem Biotechnol 195, 3096–3108 (2023). https://doi.org/10.1007/s12010-022-04267-y

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  • DOI: https://doi.org/10.1007/s12010-022-04267-y

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