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Hierarchically Structured CA@ZIF-8 Biohybrids for Carbon Dioxide Mineralization

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Abstract

Carbonic anhydrase (CA) is a powerful biocatalyst for carbon dioxide (CO2) mineralization, of which immobilization is usually used for maintaining its catalytic activity against harsh external stimuli. However, the incorporated materials for CA immobilization would commonly increase the internal diffusion resistance during the catalytic process, thereby decreasing the catalytic efficiency. In our study, poly-l-glutamic acid (PLGA) as the structure regulator was used to induce the synthesis of CA@zeolitic imidazolate framework-8 (CA@ZIF-8) biohybrids. The introduction of PLGA that could coordinate with Zn2+ interfered the crystallization of ZIF-8, thereby changing the morphological structure of CA@ZIF-8 biohybrids. With the increase of PLGA amount from 0 to 60 mg, PLGA(x)-CA@ZIF-8 biohybrids were gradually transformed from a dodecahedron structure to a 3D lamellar nano-flower structure, which caused elevated exposed surface area. Accordingly, the loading ratio was increased from 34.6 to 49.8 mg gcat−1, while the catalytic activity was elevated from 20.6 to 23.4%. The CO2 conversion rate was enhanced by nearly two folds compared to PLGA(0)-CA@ZIF-8 under the optimized condition. The final CaCO3 yield could reach 5.6 mg mgcat−1, whereas the reaction system could remain above 80% of the initial reaction activity after 8 cycles.

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Funding

This work was supported by the National Key R&D Program of China (2022YFC2105900, 2021YFC2102300), National Natural Science Fund of China (22122809), Natural Science Fund of Tianjin (19JCYBJC19700), Open Funding Project of the State Key Laboratory of Biochemical Engineering (2020KF-06) and Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project (TSBICIP-KJGG-003) for financial support.

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Authors and Affiliations

  1. School of Environmental Science & Engineering, Tianjin University, Tianjin, 300072, People’s Republic of China

    Zhuo Wang, Yang Zhao, Zhenhua Wu, Jiaxu Zhang, Boyu Zhang, Han Wang & Jiafu Shi

  2. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965/161, Tehran, Iran

    Zolfaghari Emameh Reza

  3. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 10090, People’s Republic of China

    Jiafu Shi

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  1. Zhuo Wang
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  2. Yang Zhao
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  3. Zhenhua Wu
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  5. Boyu Zhang
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  6. Han Wang
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Contributions

Jiafu Shi, Zhuo Wang, and Yang Zhao contributed to the study conception and design. Formal analysis was finished by Yang Zhao, Zhuo Wang, Zhenhua Wu, Jiaxu Zhang, Boyu Zhang, Han Wang, and Zolfaghari Emameh Reza. Investigation, methodology, and data curation were performed by Zhuo Wang. Conceptualization, supervision, project administration, review, and editing were in charge of Jiafu Shi. The first draft of the manuscript was written by Zhuo Wang. All authors read and approved the final manuscript.

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Correspondence to Jiafu Shi.

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Wang, Z., Zhao, Y., Wu, Z. et al. Hierarchically Structured CA@ZIF-8 Biohybrids for Carbon Dioxide Mineralization. Appl Biochem Biotechnol 195, 2829–2842 (2023). https://doi.org/10.1007/s12010-022-04250-7

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