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Antifungal Activity of Camelus-Derived LFA-LFC Chimeric Peptide Gelatin Film and Effect on Oral Bacterial Biofilm

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Abstract

The objective of this study was to construct lactoferrin (LFA-LFC) chimeric peptide gelatin drug-loaded fiber film by tissue engineering strategy, and study its bacteriostatic effect on oral pathogens (especially Candida albicans) and its effect on biofilm. First of all, LFA-LFC chitosan nanoparticles were prepared firstly, and then fluconazole gelatin (LF/GH/F) film loaded with LFA-LFC was prepared by electrospinning. Scanning electron microscope (SEM), mechanical strength, drug release, cytotoxicity, and real-time PCR were used to test the properties of the synthesized materials. SEM showed that there was the reticular structure for fiber film before and after cross-linking. LF/GH/F film had no obvious cytotoxicity, with good biocompatibility and drug release; real-time PCR and antibacterial test showed that the LF/GH/F film had good antibacterial activity. LF/GH/F film has a good inhibitory effect on oral pathogens, and its mechanism is related to biofilm. The antibacterial experiments of nanofiber membrane in vitro and the effect of bacterial biofilm were carried out. The effect of LF/GH/F on oral microbial flora structure was studied by fluorescence quantitative pest techniques.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

The authors are thankful to the higher authorities for the facilities provided.

Funding

This work was supported by grants from the Postdoctoral Science Foundation of China (2018M631887).

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Authors and Affiliations

  1. Department of Oral Medicine, School and Hospital of Stomatology, Jilin University, Changchun, 130021, People’s Republic of China

    Chen Li, Yang Zhou, Kai Kang & Yan Ge

  2. State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, People’s Republic of China

    Chen Li

  3. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun, 130021, People’s Republic of China

    Dandan Wang

  4. Department of Oral Pathology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, People’s Republic of China

    Dandan Wang

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Li, C., Wang, D., Zhou, Y. et al. Antifungal Activity of Camelus-Derived LFA-LFC Chimeric Peptide Gelatin Film and Effect on Oral Bacterial Biofilm. Appl Biochem Biotechnol 195, 2993–3010 (2023). https://doi.org/10.1007/s12010-022-04248-1

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