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Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus

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Abstract

Lignocellulose in maize straw includes cellulose, hemicellulose, and lignin, and the degradation of lignocellulose is a complex process in which multiple enzymes are jointly involved. In exploring the co-degradation of a certain substrate by multiple enzymes, different enzymes are combined freely for the achievement of the effective synergism. Additionally, some organic acids and small molecule aromatic compounds can also increase the enzymatic activity of lignin enzymes and improve the degradation rate of lignin. In this study, manganese peroxidase (MnP) from Irpex lacteus (I. lacteus) was heterologously expressed in food-grade Schizosaccharomyces pombe (S. pombe). The multiple enzymes co-fermentation conditions were initially screened by orthogonal tests: 0.5% CaCl2, 1% 10,000 U/g Laccase (Lac), 0.3% MnSO4, and 0.4% glucose oxidase (GOD). It was showed that the lignin degradation rate could reach 65.85% after 3 days of synergistic degradation with the addition of 0.02% Tween-80, 0.5 mM oxalic acid. This indicates that oxalic acid has a promoting effect on the activity of MnP, and the promoting effect is more significant when Tween-80 is complexed with oxalic acid.

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The [DATA TYPE] data used to support the findings of this study are available from the corresponding author upon request.

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Funding

This study was financially supported by the national key research and development program (2021YFC2103004, 2021YFA0910402), by the Open Funding Project of the State Key Laboratory of Biochemical Engineering, China (2018KF-02), and by Key Research and Development Program (Social Development) of Zhenjiang City (SH2020021).

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Authors and Affiliations

  1. School of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, China

    Huayou Chen, Shouzhi Li, Zhoulei Cui, Tao Feng, Hongcheng Wang, Zhong Ni, Ebin Gao & Zhen Fang

  2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 10090, China

    Huayou Chen

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  1. Huayou Chen
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All authors contributed to the study conception and design. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. All authors agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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Correspondence to Huayou Chen or Hongcheng Wang.

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Chen, H., Li, S., Cui, Z. et al. Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus. Appl Biochem Biotechnol 195, 3855–3871 (2023). https://doi.org/10.1007/s12010-022-04189-9

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