Skip to main content
SpringerLink
Log in

Isolation, Characterization, Antioxidant, and Wound Healing Activities of Extracellular Polysaccharide from Endophytic Fungus Talaromyces purpureogenus

  • Original Article
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, two extracellular polysaccharides (TEPS1 and TEPS2) were isolated from the endophytic fungus (Talaromyces purpureogenus) and purified by DEAE-Sepharose Fast Flow column using NaCl as gradient eluent. The HPLC analysis displayed that TEPS1 was composed of mannose (38.70%), ribose (25.02%), glucose (19.34%), and galactose (16.94%) while the TEPS2 composed by mannose (100%). The NMR results indicated that TEPS1 exhibited α-glycosidic configurations. The both polysaccharides, TEPS1 and TEPS2 were exhibited a good antioxidant activity in terms of DPPH, ABTS, and •OH scavenging. However, TEPS1 showed a higher antioxidant activity than TEPS2. The IC50 of TEPS1 were 32.16, 192.57, and 54.67 μg·mL−1, for DPPH, ABTS, and •OH radical scavenging, respectively. Furthermore, TEPS1 showed the high cellular antioxidant and wound healing activity in the human embryonic kidney (HEK293) cell line. Overall, these two polysaccharides were promising in antioxidant activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Data Availability

The data generated and analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Liang, X.-X., Gao, Y.-Y., Pan, Y., Zou, Y.-F., He, M., He, C.-L., Li, L.-X., Yin, Z.-Q., & Lv, C. (2019). Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii. Carbohydrate Polymers, 203, 45–51.

    Article  CAS  PubMed  Google Scholar 

  2. Kungel, P. T. A. N., Correa, V. G., Corrêa, R. C. G., Peralta, R. A., Soković, M., Calhelha, R. C., Bracht, A., Ferreira, I. C. F. R., & Peralta, R. M. (2018). Antioxidant and antimicrobial activities of a purified polysaccharide from yerba mate (Ilex paraguariensis). International Journal of Biological Macromolecules, 114, 1161–1167.

    Article  CAS  PubMed  Google Scholar 

  3. Li, H., Cao, K., Cong, P., Liu, Y., Cui, H., & Xue, C. (2018). Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae. Carbohydrate Polymers, 190, 87–94.

    Article  CAS  PubMed  Google Scholar 

  4. Yang, J. P., Hsu, T., Lin, F., Hsu, W., & Chen, Y. (2012). Potential antidiabetic activity of extracellular polysaccharides in submerged fermentation culture of Coriolus versicolor LH1. Carbohydrate Polymers, 90, 174–180.

    Article  CAS  PubMed  Google Scholar 

  5. Wang, Z., Xue, R., Cui, J., Wang, J., Fan, W., Zhang, H., & Zhan, X. (2019). Antibacterial activity of a polysaccharide produced from Chaetomium globosum CGMCC 6882. International Journal of Biological Macromolecules, 125, 376–382.

    Article  CAS  PubMed  Google Scholar 

  6. Yu, W., Chen, G., Zhang, P., & Chen, K. (2016). Purification, partial characterization and antitumor effect of an exopolysaccharide from Rhizopus nigricans. International Journal of Biological Macromolecules, 82, 299–307.

    Article  CAS  PubMed  Google Scholar 

  7. Freitas, F., Torres, C. A. V., & Reis, M. A. M. (2017). Engineering aspects of microbial exopolysaccharide production. Bioresource Technology, 245, 1674–1683.

    Article  CAS  PubMed  Google Scholar 

  8. Wang, Y., Li, Y., Li, S., Li, Q., Fan, W., Kiatoukosin, L., & Chen, J. (2019). Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties. International Journal of Biological Macromolecules, 133, 172–183.

    Article  CAS  PubMed  Google Scholar 

  9. Li, S.-C., Yang, X.-M., Ma, H.-L., Yan, J.-K., & Guo, D.-Z. (2015). Purification, characterization and antitumor activity of polysaccharides extracted from Phellinus igniarius mycelia. Carbohydrate Polymers, 133, 24–30.

    Article  CAS  PubMed  Google Scholar 

  10. Kumari, M., Taritla, S., Sharma, A., & Jayabaskaran, C. (2018). Antiproliferative and antioxidative bioactive compounds in extracts of marine-derived endophytic fungus Talaromyces purpureogenus. Frontiers in Microbiology, 9, 1777.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Wang, W., Wan, X., Liu, J., Wang, J., Zhu, H., Chen, C., & Zhang, Y. (2018). Two new terpenoids from Talaromyces purpurogenus. Mar Drugs, 16(5), 150.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Sahu, M. K., Kaushik, K., Das, A., & Jha, H. (2020). In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2. Bioresources and Bioprocessing, 7, 14.

    Article  Google Scholar 

  13. Masuko, T., Minami, A., Iwasaki, N., Majima, T., Nishimura, S.-I., & Lee, Y. C. (2005). Carbohydrate analysis by a phenol–sulfuric acid method in microplate format. Analytical Biochemistry, 339, 69–72.

    Article  CAS  PubMed  Google Scholar 

  14. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

    Article  CAS  PubMed  Google Scholar 

  15. Mariadoss, A. V., Park, S., Saravanakumar, K., Sathiyaseelan, A., & Wang, M.-H. (2021). Ethyl Acetate fraction of Helianthus tuberosus L. induces anti-diabetic, and wound-healing activities in insulin-resistant human liver cancer and mouse fibroblast cells. Antioxidants, 10(1), 99.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Cai, L., Zou, S., Liang, D., & Luan, L. (2018). Structural characterization, antioxidant and hepatoprotective activities of polysaccharides from Sophorae tonkinensis Radix. Carbohydrate Polymers, 184, 354–365.

    Article  CAS  PubMed  Google Scholar 

  17. An, L., Si, C., Wang, G., Sui, W., & Tao, Z. (2019). Enhancing the solubility and antioxidant activity of high-molecular-weight lignin by moderate depolymerization via in situ ethanol/acid catalysis. Industrial Crops and Products, 128, 177–185.

    Article  CAS  Google Scholar 

  18. Saravanakumar, K., Park, S., Sathiyaseelan, A., Kim, K.-N., Cho, S.-H., Mariadoss, A. V., & Wang, M.-H. (2021). Metabolite profiling of methanolic extract of Gardenia jaminoides by LC-MS/MS and GC-MS and its anti-diabetic, and anti-oxidant activities. Pharmaceuticals, 14(2), 102.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Hu, X., Saravanakumar, K., Jin, T., & Wang, M.-H. (2019). Mycosynthesis, characterization, anticancer and antibacterial activity of silver nanoparticles from endophytic fungus Talaromyces purpureogenus. International Journal of Nanomedicine, 14, 3427–3438.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Saravanakumar, K., Vivek, R., Sithranga Boopathy, N., Yaqian, L., Kathiresan, K., & Chen, J. (2015). Anticancer potential of bioactive 16-methylheptadecanoic acid methyl ester derived from marine Trichoderma. Journal of Applied Biomedicine, 13, 199–212.

    Article  Google Scholar 

  21. Shi, L. (2016). Bioactivities, isolation and purification methods of polysaccharides from natural products: A review. International journal of biological macromolecules, 92, 37–48.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Zhang, M., Wang, G., Lai, F., & Wu, H. (2016). Structural characterization and immunomodulatory activity of a novel polysaccharide from Lepidium meyenii. Journal of Agricultural and Food Chemistry, 64, 1921–1931.

    Article  CAS  PubMed  Google Scholar 

  23. Huang, S.-Q., Li, J.-W., Li, Y.-Q., & Wang, Z. (2011). Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum. International Journal of Biological Macromolecules, 48, 165–169.

    Article  CAS  PubMed  Google Scholar 

  24. Wang, F., Wang, W., Huang, Y., Liu, Z., & Zhang, J. (2015). Characterization of a novel polysaccharide purified from a herb of Cynomorium songaricum Rupr. Food Hydrocolloids, 47, 79–86.

    Article  CAS  Google Scholar 

  25. Li, B., Zhang, N., Wang, D. X., Jiao, L., Tan, Y., Wang, J., Li, H., Wu, W., & Jiang, D. C. (2018). Structural analysis and antioxidant activities of neutral polysaccharide isolated from Epimedium koreanum Nakai. Carbohydrate Polymers, 196, 246–253.

    Article  CAS  PubMed  Google Scholar 

  26. Ye, L., Zhang, J., Yang, Y., Zhou, S., Liu, Y., Tang, Q., Du, X., Chen, H., & Pan, Y. (2009). Structural characterisation of a heteropolysaccharide by NMR spectra. Food Chemistry, 112, 962–966.

    Article  CAS  Google Scholar 

  27. Xiu, A., Kong, Y., Zhou, M., Zhu, B., Wang, S., & Zhang, J. (2010). The chemical and digestive properties of a soluble glucan from Agrobacterium sp. ZX09. Carbohydrate Polymers, 82, 623–628.

    Article  CAS  Google Scholar 

  28. Wang, X., Zhang, M., Zhang, D., Wang, X., Cao, H., Zhang, Q., & Yan, C. (2019). Structural elucidation and anti-osteoporosis activities of polysaccharides obtained from Curculigo orchioides. Carbohydrate Polymers, 203, 292–301.

    Article  CAS  PubMed  Google Scholar 

  29. Zeng, Y.-J., Yang, H.-R., Ou, X.-Y., Su, H.-H., Zong, M.-H., Yang, J.-G., & Lou, W.-Y. (2019). Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities. International Journal of Biological Macromolecules, 141, 460–470.

    Article  CAS  PubMed  Google Scholar 

  30. Sharma, O. P., & Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food Chemistry, 113, 1202–1205.

    Article  CAS  Google Scholar 

  31. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237.

    Article  CAS  PubMed  Google Scholar 

  32. Reiter, R. J., Melchiorri, D., Sewerynek, E., Poeggeler, B., Barlow-Walden, L., Chuang, J., Ortiz, G. G., & AcuñaCastroviejo, D. (1995). A review of the evidence supporting melatonin’s role as an antioxidant. Journal of Pineal Research, 18, 1–11.

    Article  CAS  PubMed  Google Scholar 

  33. Koppenol, W. H. (1993). The centennial of the Fenton reaction. Free Radical Biology and Medicine, 15, 645–651.

    Article  CAS  PubMed  Google Scholar 

  34. Joanna, K., Michał, P. & Anna, P. (2017). Osmotic properties of polysaccharides solutions. Solubility of Polysaccharides, 23. https://doi.org/10.5772/intechopen.69864

  35. Birnboim, H. C. (1986). DNA strand breaks in human leukocytes induced by superoxide anion, hydrogen peroxide and tumor promoters are repaired slowly compared to breaks induced by ionizing radiation. Carcinogenesis, 7, 1511–1517.

    Article  CAS  PubMed  Google Scholar 

  36. Dogan,K.H., (2019). In Wound Healing-Current Perspectives, IntechOpen. 260p

Download references

Funding

This work was supported by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017H1D3A1A01052610), National Research Foundation of Korea (2019R1A1055452) and Korea Basic Science Institute (C210300).

Author information

Author notes

  1. Xiaowen Hu and Kandasamy Saravanakumar equally contributed to this work. 

Authors and Affiliations

  1. Department of Bio-Health Convergence, Kangwon National University, Chuncheon, 200-701, South Korea

    Xiaowen Hu, Kandasamy Saravanakumar, Ki-seok Han & Myeong-Hyeon Wang

  2. Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon, 24341, Republic of Korea

    SeonJu Park

Authors
  1. Xiaowen Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kandasamy Saravanakumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. SeonJu Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ki-seok Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Myeong-Hyeon Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Xiaowen Hu: data curation, formal analysis, investigation, methodology, writing—review and editing. Kandasamy Saravanakumar: conceptualization, data curation, formal analysis, investigation, methodology, visualization, writing—original draft, writing—review and editing. SeonJu Park: formal analysis, data curation. Ki-seok Han: formal analysis, investigation. Myeong-Hyeon Wang: project administration, resources, supervision, validation, writing—review and editing.

Corresponding author

Correspondence to Myeong-Hyeon Wang.

Ethics declarations

Ethics Approval

This article does not contain any studies with animals or human participants.

Consent to Participate

The authors agreed to participate in this work.

Consent for Publication

The authors agreed to publish this work.

Conflict of Interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOC 396 KB)

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hu, X., Saravanakumar, K., Park, S. et al. Isolation, Characterization, Antioxidant, and Wound Healing Activities of Extracellular Polysaccharide from Endophytic Fungus Talaromyces purpureogenus. Appl Biochem Biotechnol 195, 3822–3839 (2023). https://doi.org/10.1007/s12010-022-04187-x

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-022-04187-x

Keywords

Search

Navigation