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Anti-urolithiatic Activity of Daidzin in Ethylene Glycol-Induced Urolithiasis in Rats

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Abstract

Urolithiasis is a common urological disorder, which causes considerable morbidity in both genders at all age groups worldwide. Though treatment options such as diuretics and non-invasive techniques to disintegrate the deposits are available, but often they are found less effective in the clinics. In this work, we planned to investigate the ameliorative effects of daidzin against the ethylene glycol (EG)-induced urolithiasis in rats. The male albino rats were distributed into four groups (n = 6) as control (group I), urolithiasis induced by the administration of 0.75% EG (group II), urolithiasis induced rats treated with 50 mg/kg of daidzin (group III), and urolithiasis rats treated with standard drug 750 mg/kg of cystone (group IV). The urine volume, pH, and total protein in the urine were assessed. The activities of marker enzymes in both plasma and kidney tissues were analyzed using assay kits. The levels of kidney function markers such as calcium, oxalate, urea, creatinine, uric acid, magnesium, BUN, and phosphorous were estimated using assay kits. The status of antioxidants and inflammatory cytokines were also examined using kits. The renal tissues were examined by histopathological analysis. Our results revealed that the daidzin treatment effectively decreased the urine pH and protein level and increased the urine volume in the urolithiasis rats. Daidzin decreased the calcium, oxalate, uric acid, and urea, creatinine, and BUN levels and also improved the magnesium and phosphorus in the urolithiasis rats. The activities of AST, ALT, ALP, GGT, and LDH were effectively reduced by the daidzin in both serum and renal tissue. Daidzin also reduced the inflammatory marker and increased the antioxidant levels. Histopathology results also proved the therapeutic effects of daidzin. Together, our results displayed that daidzin is effective in the amelioration of EG-induced urolithiasis in rats.

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Abbreviations

EG:

Ethylene glycol

CKD:

Chronic kidney disease

ESRD:

End-stage renal disease

AST:

Aspartate transaminase

ALT:

Alanine transaminase

ALP:

Alkaline phosphatase

GGT:

Gamma glutamyl transferase (GGT)

LDH:

Lactate dehydrogenase

KCl:

Potassium chloride (KCl)

References

  1. Khan, S., et al. (2016). Kidney stones. Nature Reviews Disease Primers, 2(1), 1–23.

    Article  Google Scholar 

  2. Robertson, W. G. (2003). Renal stones in the tropics. Seminars in Nephrology, 23(1), 77–87.

    Article  Google Scholar 

  3. Wathigo, F. K., Hayombe, A., & Maina, D. (2017). Urolithiasis analysis in a multiethnic population at a tertiary hospital in Nairobi. Kenya. BMC Res Notes, 10, 158. https://doi.org/10.1186/s13104-017-2474-3

    Article  CAS  Google Scholar 

  4. López, M., & Hoppe, B. (2010). History, epidemiology and regional diversities of urolithiasis. Pediatric Nephrology, 25(1), 49–59.

    Article  Google Scholar 

  5. Strope, S. A., Stuart Wolf, J., & Hollenbeck, B. K. (2010). Changes in gender distribution of urinary stone disease. Urology, 75(3), 543-546.e1.

    Article  Google Scholar 

  6. Shah, J., & Whitfield, H. (2002). Urolithiasis through the ages. BJU International, 89(8), 801–810.

    Article  Google Scholar 

  7. Worcester, E., & Coe, F. (2008). Nephrolithiasis. Primary Care: Clinics in Office Practice, 35(2), 369–391.

    Article  Google Scholar 

  8. Jawalekar, S., Surve, V., & Bhutey, A. (2010). The composition and quantitative analysis of urinary calculi in patients with renal calculi. Nepal Medical College Journal, 12(3), 145–148.

    CAS  Google Scholar 

  9. El-Zoghby, Z., et al. (2012). Urolithiasis and the risk of ESRD. Clinical Journal of the American Society of Nephrology, 7(9), 1409–1415.

    Article  Google Scholar 

  10. Zisman, A. (2017). Effectiveness of treatment modalities on kidney stone recurrence. Clinical Journal of the American Society of Nephrology, 12(10), 1699–1708.

    Article  CAS  Google Scholar 

  11. Dharmalingam, S., et al. (2014). Anti-urolithiatic activity of Melia azedarach Linn leaf extract in ethylene glycol-induced urolithiasis in male albino rats. Tropical Journal of Pharmaceutical Research, 13(3), 391–397.

    Article  Google Scholar 

  12. Kant, R., Singh, T., & Singh, S. (2020). Mechanistic approach to herbal formulations used for urolithiasis treatment. Obesity Medicine, 19, 100266.

    Article  Google Scholar 

  13. York, N., Borofsky, M., & Lingeman, J. (2015). Risks associated with drug treatments for kidney stones. Expert Opinion on Drug Safety, 14(12), 1865–1877.

    Article  CAS  Google Scholar 

  14. Pareta, S., Patra, K., Mazumder, P., & Sasmal, D. (2011). Establishing the principle of herbal therapy for antiurolithiatic activity: A review. Journal of Pharmacology and Toxicology, 6(3), 321–332.

    Article  Google Scholar 

  15. Erickson, S., Vrtiska, T., & Lieske, J. (2011). Effect of Cystone® on urinary composition and stone formation over a one year period. Phytomedicine, 18(10), 863–867.

    Article  CAS  Google Scholar 

  16. Pongkitwitoon, B., Sakamoto, S., & Tanaka, H. (2009). Enzyme-linked immunosorbent assay for total isoflavonoids in Pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies. Planta Medica, 76(8), 831–836.

    Article  Google Scholar 

  17. Jin, W., Tan, Y., Chen, Y., & Wang, Y. (2003). Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC. Zhongguo Zhong Yao Za Zhi (in Chinese), 28(1), 49–51.

    CAS  Google Scholar 

  18. Zhou, H., Wang, J., & Yan, F. (2007). Separation and determination of puerarin, daidzin and daidzein in stems and leaves of Pueraria thomsonii by RP-HPLC. Zhongguo Zhong Yao Za Zhi (in Chinese), 32(10), 937–939.

    CAS  Google Scholar 

  19. Park, E., et al. (2006). Intestinal bacteria activate estrogenic effect of main constituents puerarin and daidzin of Pueraria thunbergiana. Biological & Pharmaceutical Bulletin, 29(12), 2432–2435.

    Article  CAS  Google Scholar 

  20. Osman, S., & Fett, W. (1983). Isoflavone glucoside stress metabolites of soybean leaves. Phytochemistry, 22(9), 1921–1923.

    Article  CAS  Google Scholar 

  21. Rha, C., et al. (2019). Simple and efficient production of highly soluble daidzin glycosides by amylosucrase from Deinococcus geothermalis. Journal of Agricultural and Food Chemistry, 67(46), 12824–12831.

    Article  CAS  Google Scholar 

  22. Keung, W., & Vallee, B. (1998). Daidzin and its antidipsotropic analogs inhibit serotonin and dopamine metabolism in isolated mitochondria. Proceedings of the National Academy of Sciences, 95(5), 2198–2203.

    Article  CAS  Google Scholar 

  23. Yagasaki, K. (2019). Phytochemicals, their intestinal metabolites, and skeletal muscle function (pp. 421–438). Academic Press.

    Google Scholar 

  24. Yasuda, T., Kano, Y., Saito, K., & Iohsawa, K. (1994). Urinary and biliary metabolites of daidzin and daidzein in rats. Biological and Pharmaceutical Bulletin, 17(10), 1369–1374.

    Article  CAS  Google Scholar 

  25. Cloutier, J., Villa, L., Traxer, O., & Daudon, M. (2015). Kidney stone analysis: “Give me your stone, I will tell you who you are! World Journal of Urology, 33(2), 157–169.

    Article  Google Scholar 

  26. Alelign, T., & Petros, B. (2018). Kidney stone disease: An update on current concepts. Advances in Urology., 2018, 1–12.

    Article  Google Scholar 

  27. Tasian, G., Kabarriti, A., Kalmus, A., & Furth, S. (2017). Kidney stone recurrence among children and adolescents. The Journal of Urology, 197(1), 246–252.

    Article  Google Scholar 

  28. Orhan, N., Onaran, M., Sen, L., & Gonul, L. (2015). Preventive treatment of calcium oxalate crystal deposition with immortal flowers. Journal of Ethnopharmacology, 163, 60–67.

    Article  Google Scholar 

  29. Karadi, R. V., Gadge, N. B., Alagawadi, K. R., & Savadi, R. V. (2006). Effect of Moringaoleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats. Journal of Ethnopharmacology., 105, 306–311.

    Article  Google Scholar 

  30. Selvam, R., Kalaiselvi, P., Govindaraj, A., BalaMurugan, V., & Sathish Kumar, A. S. (2001). Effect of A. lanata leaf extract and vediuppuchunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria. Pharmacological Research, 43, 89–93.

    Article  CAS  Google Scholar 

  31. Evan, A. P., Worcester, E. M., Coe, F. L., Williams, J., & Lingeman, J. E. (2015). Mechanisms of human kidney stone formation. Urolithiasis., 43(Suppl. 1), 19–32.

    Article  Google Scholar 

  32. Huang, H. S., Ma, M. C., Chen, C. F., & Chen, J. (2003). Lipid peroxidation and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology, 62, 1123–1128.

    Article  Google Scholar 

  33. Khan, S. R. (2013). Reactive oxygen species as the molecular modulators of calcium oxalate kidney stone formation: Evidence from clinical and experimental investigations. Journal of Urology, 189, 803–811.

    Article  CAS  Google Scholar 

  34. Dwiecki, K., Neunert, G., Polewski, P., & Polewski, K. (2009). Antioxidant activity of daidzein, a natural antioxidant, and its spectroscopic properties in organic solvents and phosphatidylcholine liposomes. Journal of Photochemistry and Photobiology B: Biology, 96(3), 242–248.

    Article  CAS  Google Scholar 

  35. Khan, S. R., Canales, B. K., & Dominguez-Gutierrez, P. R. (2021). Randall’s plaque and calcium oxalate stone formation: Role for immunity and inflammation. Nature Reviews. Nephrology, 17(6), 417–433.

    Article  CAS  Google Scholar 

  36. Umekawa, T., Chegini, N., & Khan, S. R. (2002). Oxalate ions and calcium oxalate crystals stimulate MCP-1 expression by renal epithelial cells. Kidney International, 61, 105–112.

    Article  CAS  Google Scholar 

  37. Aihara, K., Byer, K. J., & Khan, S. R. (2003). Calcium phosphate-induced renal epithelial injury and stone formation: Involvement of reactive oxygen species. Kidney International, 64, 1283–1291.

    Article  CAS  Google Scholar 

  38. Wang, Z. V., & Scherer, P. E. (2016). Adiponectin, the past two decades. Journal of Molecular Cell Biology, 8, 93–100.

    Article  CAS  Google Scholar 

  39. Hao, R., Su, G., Sun, X., et al. (2019). Adiponectin attenuates lipopolysaccharide-induced cell injury of H9c2 cells by regulating AMPK pathway. Acta Biochimica et Biophysica Sinica (Shanghai), 51, 168–177.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Urology, Shanxi Bethune Hospital, Taiyuan, 030032, China

    Shuai Yuan & Ruimin Ren

  2. Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia

    Ibrahim Abdel Aziz Ibrahim

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  1. Shuai Yuan
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  2. Ibrahim Abdel Aziz Ibrahim
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Yuan, S., Ibrahim, I.A.A. & Ren, R. Anti-urolithiatic Activity of Daidzin in Ethylene Glycol-Induced Urolithiasis in Rats. Appl Biochem Biotechnol 195, 905–918 (2023). https://doi.org/10.1007/s12010-022-04124-y

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