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Polysaccharides of Weissella cibaria Act as a Prebiotic to Enhance the Probiotic Potential of Lactobacillus rhamnosus

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Abstract

This work aimed to investigate the effect of EPS (extracellular polysaccharide) of Weissella cibaria as a prebiotic to promote the growth and antibacterial properties of Lactobacillus rhamnosus. The morphological, growth behavior, and antibacterial properties of L. rhamnosus were determined in MRSB (de Man Rogosa Sharpe broth) supplemented with different concentrations of EPS (0.1–2%). The results revealed that the incorporation of the EPS (2%) in MRSA improved the bacterial growth in terms of colony-forming unit (CFU, 0.7 × 105 CFU/mL) compared to L. rhamnosus grown in bare MRSA. The SEM observation revealed that EPS incorporation in the MRSB culture media does not affect the morphological properties of L. rhamnosus. Moreover, it was confirmed that the extract of probiotics cultured in MRSA supplemented with EPS (2%) was exhibited strong antibacterial and antibiofilm activity against targeted pathogens. This L. rhamnosus extract was found to be biocompatible evidanced by erythrocyte hemolysis assay. These results confirmed that EPS regulates the growth of probiotics, resists pathogen infection, and biocompatibility.

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Data Availability

The data generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017H1D3A1A01052610), National Research Foundation of Korea (2019R1A1055452).

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Author notes

  1. Soyoung Park and Kandasamy Saravanakumar equally contributed to this work.

Authors and Affiliations

  1. Department of Bio Health Convergence, Kangwon National University, Chuncheon, 200-701, South Korea

    Soyoung Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, Ki-Seok Han & Myeong-Hyeon Wang

  2. Kangwon Center for Systems Imaging, Chuncheon, 24341, Republic of Korea

    Jooeun Lee

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  1. Soyoung Park
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  2. Kandasamy Saravanakumar
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  3. Anbazhagan Sathiyaseelan
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Contributions

Soyoung Parka: formal analysis, investigation, methodology, writing—original draft. Kandasamy Saravanakumar: conceptualization, data curation, formal analysis, investigation, methodology, visualization, writing—original draft, writing—review and editing. Anbazhagan Sathiyaseelan: formal analysis, data curation. Ki-seok Han: formal analysis, investigation. Jooeun Lee: investigation, methodology. Myeong-Hyeon Wang: project administration, resources, supervision, validation, writing—review and editing.

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Correspondence to Myeong-Hyeon Wang.

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Park, S., Saravanakumar, K., Sathiyaseelan, A. et al. Polysaccharides of Weissella cibaria Act as a Prebiotic to Enhance the Probiotic Potential of Lactobacillus rhamnosus. Appl Biochem Biotechnol 195, 3928–3940 (2023). https://doi.org/10.1007/s12010-022-04104-2

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