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Cardioprotective Role of Swertiamarin, a Plant Glycoside Against Experimentally Induced Myocardial Infarction via Antioxidant and Anti-inflammatory Functions

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Abstract

The study examined the protective effects of swertiamarin on rats with experimentally induced myocardial infarction. Three to six week-old male albino Wistar rats were used in this study and experimental myocardial infarction (MI) was induced using isoproterenol. Our results showed that swertiamarin restored the alteration in heart weight, body weight, and heart weight/tibia length ratio of MI-induced rats to basal levels significantly (p < 0.05). Swertiamarin significantly (p < 0.05) restored the levels of cardiac pathophysiological marker creatine kinase (CKMB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine transaminase (ALT), and cardiac troponin I (cTn-1) to near normalcy in MI-induced rats. Levels of oxidative stress markers malondialdehyde (MDA), protein carbonyls (PC), and levels of Vitamin C and Vitamin E were significantly (p < 0.05) reverted to near basal levels in MI-induced rats by swertiamarin. Levels of the antioxidant glutathione (GSH) and antioxidant enzymes which include superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-s-transferase (GST), glutathione reductase (GR), and plasma total antioxidant capacity (TAC) were (p < 0.05) brought to near normalcy in MI-induced rats by swertiamarin. Levels of sodium (Na), potassium (k), and calcium (Ca) ATPases were significantly (p < 0.05) restored to near normalcy in MI-induced rats by swertiamarin. Status of pro-inflammatory cytokines including tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and histological aberrations were also significantly (p < 0.05) restored to near normalcy in MI-induced rats by swertiamarin. Together, our results concluded that swertiamarin exerts significant cardioprotective functions in experimental MI in rats.

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Abbreviations

pH:

Potential of hydrogen

M:

Molar

mg:

Milligram

µg:

Microgram

kg:

Kilogram

ISO:

Isoproterenol hydrochloride

MI:

Myocardial infarction

CK-MB:

Creatine kinase

LDH:

Lactate dehydrogenase

AST:

Aspartate aminotransferase

ALT:

Alanine transaminase

cTn 1:

Cardiac troponin I

GSH:

Glutathione

SOD:

Superoxide dismutase

CAT:

Catalase

GPx:

Glutathione peroxidase

GST:

Glutathione-s-transferase

GR:

Glutathione reductase

TAC:

Plasma total antioxidant capacity plasma

Na:

Sodium (Na)

k:

Potassium

Ca:

Calcium

TNF-α:

Tumor necrosis factor

IL-6:

Interleukin-6

BSA:

Bovine serum albumin

NaCl:

Sodium chloride

ISO:

Isoproterenol hydrochloride

MDA:

Malondialdehyde

PC:

Protein carbonyls

Vit-C:

Vitamin C

Vit-E:

Vitamin E

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Authors and Affiliations

  1. Department of Cardiology, Shandong Provincial Third Hospital, Shandong University, Jinan, 250031, Shandong, China

    Tao Wang, Shubin Wu & Leilei Fan

  2. Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia

    Ibrahim Abdel Aziz Ibrahim

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Wang, T., Wu, S., Ibrahim, I.A.A. et al. Cardioprotective Role of Swertiamarin, a Plant Glycoside Against Experimentally Induced Myocardial Infarction via Antioxidant and Anti-inflammatory Functions. Appl Biochem Biotechnol 195, 5394–5408 (2023). https://doi.org/10.1007/s12010-022-04094-1

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