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In Vitro Inhibitory Effect of Some Secondary Metabolites Extract from Ocimum basilicum and Salvia officinalis Against Fusarium oxysporum f.sp albedinis of Phoenix dactylifera. L

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Abstract

Fusarium of date palm is a vascular fusariosis caused by a telluric fungus Fusarium oxysporum f.sp. albedinis (F.O.A) which affects the best date varieties in Algeria. Several medicinal plants are adapted with the climatic complexity of the Saharan region, among these plants in the south-west of Algeria Salvia officinalis and Ocimum basilicum. The aim of this work is to study the antifungal effect of flavonoid extracts from Salvia officinalis and Ocimum basilicum leaves by the technique described by Bouchelta et al. (2005), as well the antifungal effect of the aqueous extract of these plants against Fusarium oxysporum f.sp. albedinis isolated from the grove of Igli. The evaluation of the antifungal activity revealed a slight mycelial growth anti-proportional to the concentration of flavonoid extract added to the culture medium with an inhibition index of 73.04% at the concentration 3.17 μg/ml and 57.42% at the concentration 1.13 µg/ml for flavonoids extract of Ocimum basilicum and Salvia officinalis, respectively. In addition, the aqueous extract revealed an inhibition index of 35.15% at the concentration of 0.33 mg/ml and 10.54% at the concentration of 3.92 mg/ml for Salvia officinalis and Ocimum basilicum, respectively.

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Data Availability

The strain used in this study was isolated from the palm grove of Igli, located in the southwest of Algeria. The isolation, purification, and identification of the pathogen with different technique components were carried out by Ms. Amina Mezouari, PhD student at the University of Béchar, 2019.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences of Nature and Life, Biology Department, University Tahri Mohammed, BP 417, Bechar, Algeria

    Nora Nahal Bouderba & Hamid Kadi

  2. Laboratory of Plant Resource Development and Food Security in Semi Arid Areas, South West of Algeria, Department of Biology, University of Bechar, Bechar, Algeria

    Nora Nahal Bouderba & Hamid Kadi

  3. Department of Hematology and Immunohematology, Omdurman Islamic University, Omdurman, Sudan

    Khalid Mohamed Khalid

  4. Omdurman Ahlia University, Faulty of Medicine, Omdurman, Sudan

    Khalid Mohamed Khalid

  5. International University of Africa, Faculty of Medical Laboratory Sciences, Khartoum, Sudan

    Khalid Mohamed Khalid

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Nahal Bouderba Nora and Kadi Hamid have done the experiments and wrote the manuscript, and Khalid Mohamed Khalid edited the manuscript.

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Correspondence to Nora Nahal Bouderba.

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Bouderba, N.N., Khalid, K.M. & Kadi, H. In Vitro Inhibitory Effect of Some Secondary Metabolites Extract from Ocimum basilicum and Salvia officinalis Against Fusarium oxysporum f.sp albedinis of Phoenix dactylifera. L. Appl Biochem Biotechnol 194, 5655–5665 (2022). https://doi.org/10.1007/s12010-022-04067-4

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