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Analysis of TCP Transcription Factors Revealed Potential Roles in Plant Growth and Fusarium oxysporum f.sp. cubense Resistance in Banana (cv. Rasthali)

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Abstract

The TCP transcription factor gene family is highly conserved among the plant species. It plays a major role in the regulation of flower symmetry, cell division, and development of leaf, fibre, and nodule in the plants by controlling the synthesis of various plant hormones. Banana is a major staple crop in the world. However, Fusarium oxysporum f. sp. cubense (Foc) infection is a major threat to banana production. The role of TCP gene family during the Foc infection is not explored till now. Herein, a total of 27 non-redundant TCP (MaTCP) gene sequences were retrieved from the banana genome and analysed for structural characteristics, phylogenetic correlation, subcellular, and chromosomal localizations. Phylogenetic analysis showed that the MaTCP proteins were highly conserved among different species and found to be the closest relative of the Oryza sativa and Zea mays. Promoter analysis of the TCP sequences showed that the cis-acting regulatory elements are associated with various stresses and environmental and hormonal signals. The higher transcript accumulation in developing tissues (fruit finger, leaves, and stem) than of mature tissues (peel and pulp) showed a significant role of MaTCP in banana (cv. Rasthali) growth and development. Further, higher expression of the certain MaTCPs in Foc race 1-infected root (MaTCP2, MaTCP4, MaTCP6) and leaf (MaTCP9 and MaTCP11) tissues of Rasthali indicated their promising role during Fusarium infection. This study will underpin the facet of TCP transcription factors on the development of biotic (Foc) stress resistance in banana.

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Acknowledgements

The authors express their gratitude to the National Agri-Food Biotechnology Institute (NABI) for research facilities and the Department of Biotechnology (DBT), Government of India, for the grant (BT/PR25789/GET/119/97/2017). The present research was also supported by the Biotechnology Industry Research Assistance Council (BIRAC) for a banana biofortification project grant. SC is thankful to the Panjab University Chandigarh, and SK is thankful to the Central University of Punjab for PhD registration. The authors would like to acknowledge DBT-eLibrary Consortium (Del-CON) for providing access to online journals.

Funding

This work was supported by the Department of Biotechnology (DBT), Government of India.

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Authors and Affiliations

  1. Plant Tissue Culture and Genetic Engineering Lab, Department of Biotechnology, Ministry of Science and Technology (Government of India), National Agri-Food Biotechnology Institute (NABI), Sector 81, Knowledge City, S.A.S. Nagar, Mohali, 140306, Punjab, India

    Siddhant Chaturvedi, Shahirina Khan & Siddharth Tiwari

  2. Department of Biotechnology, Panjab University, Chandigarh, 160014, India

    Siddhant Chaturvedi

  3. Department of Botany, Central University of Punjab, Bathinda, 151001, Punjab, India

    Shahirina Khan

  4. Department of Biotechnology, Indian Institute of Horticultural Research, Bengaluru, 560089, India

    T. R. Usharani

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  1. Siddhant Chaturvedi
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  2. Shahirina Khan
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  3. T. R. Usharani
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  4. Siddharth Tiwari
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Contributions

S.T. conceived and designed the research. S.C and SK performed computational and qRT-PCR analysis. S.T., S.C., and T.R.U. analysed the data. S.C and S.T wrote the manuscript.

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Correspondence to Siddharth Tiwari.

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12010_2022_4065_MOESM1_ESM.docx

Supplementary file1 Fig. S1: Chromosomal distribution and arrangement of MaTCP genes in Musa acuminata genome. The name represents the estimated location of genes on chromosomes. Chromosome numbers and sizes (Mb) are shown at the top and right sides, respectively. Fig. S2: Conserved motifs of MaTCP proteins. A total of 6 motifs were identified using MEME and scanned for motif enrichment using MAST. Fig. S3: Gene ontology distribution of MaTCP proteins by blast2go. GO annotation was divided into three categories: cellular component, molecular function and biological process.  Fig. S4: Expression profile of MaTCP genes under Fusarium oxysporum f. sp. cubense TR1 treated root (a) and leaf (b) tissues of banana cv. Rasthali. The expression level of all the transcripts was normalized with actin1 (GenBank Accession No. AF246288) as a housekeeping gene control. Each bar in the graph indicates the mean of three replicates with an error bar plotted as a standard deviation. The symbol * indicates significant difference at p < 0.05 with respect to unpaired student t-test , GraphPad Prism 5. (DOCX 3184 KB)

12010_2022_4065_MOESM2_ESM.docx

Supplementary file2 Table S1: qRT-PCR primers for MaTCP genes expression analysis. Table S2: Physiochemical properties of MaTCP proteins. Table S3: Promoter analysis of MaTCP genes. (DOCX 24 KB)

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Chaturvedi, S., Khan, S., Usharani, T.R. et al. Analysis of TCP Transcription Factors Revealed Potential Roles in Plant Growth and Fusarium oxysporum f.sp. cubense Resistance in Banana (cv. Rasthali). Appl Biochem Biotechnol 194, 5456–5473 (2022). https://doi.org/10.1007/s12010-022-04065-6

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  • DOI: https://doi.org/10.1007/s12010-022-04065-6

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