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Association of VEGF Gene Polymorphism (rs699947) with Glaucoma and In-Silico Study of Antiglaucoma Bioactive Compounds

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Abstract

Vascular endothelial growth factor (VEGF) is a useful expert in various maturation and other problems such as glaucoma. VEGF gene is located on the short arm of chromosome 6. It has 8 exons and 6 introns. This investigation was planned to check out the relationship of VEGF polymorphism with glaucoma patients and to cause in silico limitation of carbonic anhydrase to diminish the intraocular strain of watery humor to fix glaucoma. In this examination, a large portion of the glaucoma patients (n = 70) were males (66%) when contrasted with females (34%) as the p-value was 0.025 which showed critical outcomes and subsequently demonstrated that the sickness was more predominant in males. Glaucoma for the most part influences people between the ages of 50–60 years followed by the age bunch between 30 and 40 years. Around 36 (51.4%) were experiencing watering in the eyes, 28 (40%) had photophobia, 9 (12.8%) had a hazy cornea, 6 (8.6%) had expanded eye globe, and 15 patients (21.3%) had rosy eyes. Factual tests showed that VEGF quality SNP rs699947 had no huge relationship with glaucoma (POAG). In this study, various carbonic anhydrase inhibitor phytochemicals and synthetic chemicals were screened in silico which may be used as antiglaucoma drugs.

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Acknowledgements

I would like to acknowledge the Mughal Eye Hospital for facilitating us in blood sample collection. Special thanks to glaucoma patients without whom this research study would not be possible. I would like to acknowledge the Institute of Microbiology and Molecular Genetics, University of the Punjab, also for providing us the working facilities.

Author information

Authors and Affiliations

  1. Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan

    Nageen Hussain, Sonia Falek Sher & Muhammad Adil

  2. Department of Ophthalmology, Yantai Yuhuangding Hospital, No. 20, Yuhuangding East Road, Yantai City, 264000, Shandong Province, China

    Xuming Lin

Authors
  1. Nageen Hussain
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  2. Sonia Falek Sher
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  3. Xuming Lin
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  4. Muhammad Adil
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Contributions

1. Dr. Nageen Hussain.

Design the proposal

Experimental design

Collection of data

Performed the experiment

Wrote the paper

2. Sonia Falek Sher

Contributed data and analysis tools

Performed the analysis

3. Linxu Ming

Collection of data

Wrote the paper

Statistical analysis

4. Muhammad Adil

In silico analysis of phytochemicals

Bioinformatics data

Corresponding author

Correspondence to Xuming Lin.

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Ethics Approval

This project has been evaluated and approved by the Departmental Research Ethics and Biosafety Committee. The committee could not find any biosafety and ethical concerns related to the proposed work and its execution at the Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore.

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All the participants involved in the study had their consent.

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It is certified that the “Association of VEGF Gene Polymorphism (rs699947) with Glaucoma and In Silico Study of Antiglaucoma Bioactive Chemicals” has neither been published in any journal nor copied from anywhere else. The authors of this original article want to submit it in this journal.

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The authors declare no competing interests.

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Hussain, N., Sher, S.F., Lin, X. et al. Association of VEGF Gene Polymorphism (rs699947) with Glaucoma and In-Silico Study of Antiglaucoma Bioactive Compounds. Appl Biochem Biotechnol 194, 5185–5195 (2022). https://doi.org/10.1007/s12010-022-04014-3

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  • DOI: https://doi.org/10.1007/s12010-022-04014-3

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