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Moringa oleifera Nanoparticles Demonstrate Antifungal Activity Against Plant Pathogenic Fungi

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Abstract

Fungal diseases in plants are creating numerous problems in the developed and developing nations. Silver, a notable metal because of its inertness and its role in nanoscience, has received a considerable amount of focus in the development of an ecofriendly green solution to control many microbial infections. The herbal product from various plant sources with the combination of silver was used to develop nanoparticles, against the pathogens. In this study, we developed Moringa oleifera leaf- and flower-mediated silver nanoparticles with the particle size of 77.45 nm and 63.20 nm respectively. Fungicidal activity of both Moringa oleifera leaf (MLNp) and flower (MFNp) nanoparticles was studied in vitro against plant pathogenic fungi Pestalotiopsis mangiferae isolated from infected coconut palm. Nanoparticles from Moringa oleifera leaves and flowers reduced the radial growth of fungi significantly even at lower concentrations and acted as a potent fungistatic agent.

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Data availability

Data will be available on request.

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Not applicable

Abbreviations

AgNO3:

silver nitrate

AgNPs:

silver nanoparticles

NPs:

nanoparticles

PDA:

potato dextrose agar

nm:

nanometer

μm:

micrometer

mm:

millimeter

UV:

ultraviolet

SEM:

scanning electron microscopy

FTIR:

Fourier transform infrared spectroscopy

EtBr:

ethidium bromide

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Acknowledgements

The authors are thankful to B.S. Abdur Rahman Institute of Science and Technology, Chennai, for providing research facilities in School of Life Sciences. The authors also gratefully acknowledge the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi (ref no. DST/WOS-B/2018/1583-HFN (G)) and ASEAN University network (AUN)/Southeast Asia Engineering Education Development Network (SEED)/Japan International Cooperation Agency (JICA) SPRAC (SN042/MI.KU/2020).

Funding

This study is supported by the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi (ref no. DST/WOS-B/2018/1583-HFN (G)) and ASEAN University Network (AUN)/Southeast Asia Engineering Education Development Network (SEED)/Japan International Cooperation Agency (JICA) SPRAC (SN042/MI.KU/2020).

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Author notes

  1. Antony Jenish and S. Ranjani contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur 48, Chennai, Tamil Nadu, 600048, India

    Antony Jenish, S. Ranjani & S. Hemalatha

Authors
  1. Antony Jenish
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  2. S. Ranjani
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  3. S. Hemalatha
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Contributions

SH conceived and designed research. AJ and SR conducted the experiments. SH analyzed the data. All authors wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to S. Hemalatha.

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Jenish, A., Ranjani, S. & Hemalatha, S. Moringa oleifera Nanoparticles Demonstrate Antifungal Activity Against Plant Pathogenic Fungi. Appl Biochem Biotechnol 194, 4959–4970 (2022). https://doi.org/10.1007/s12010-022-04007-2

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