Skip to main content
Log in

GC–MS Analysis of Bioactive Compounds of Flower Extracts of Calycopteris floribunda Lam.: A Multi Potent Medicinal Plant

  • Original Article
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Calycopteris floribunda Lam. is a potent medicinal woody climber that belongs to Combretaceae. This plant is usually found in dry deciduous tropical forests and is used in various medicinal practices like Ayurveda, Unani and Sidda. Whole plant and its different parts like leaves, flowers and stem are used in the treatment of diarrhoea, dysentery, jaundice and malaria. It is also have anthelminthic, anti-inflammatory, antifungal, hepatoprotective and anticancerous activities. Knowing its medicinal properties, the present study is undertaken to investigate the preliminary phytochemical constituents and bioactive compounds of flower extracts by GC–MS. GC–MS analysis of flower extracts revealed the presence of over all 41 compounds, of which, acetone and ethanol extracts showed the presence of 13 compounds each, chloroform extract 8 and petroleum ether extract 7 compounds. Some compounds were common in two and three extracts only. The significant bioactive compounds identified are 1,2-benzenedicarboxylic acid (59.81%) in chloroform extract, triterpene lupeol (34.98%) in ethanol extract, tetratetracontane (26.99%) in petroleum ether extract and gamma sitosterol (22.04%) in acetone extract.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

All data generated or analyzed during this study are included in this article.

References

  1. Bhat, P., Hegde, G., & Hegde, G. R. (2012). Ethnomedicinal practices in different communities of Uttara Kannada district of Karnataka for treatment of wounds. Journal of ethnopharmacology, 143(2), 501–514.

    Article  PubMed  Google Scholar 

  2. Nagannawar, A. G., & Jayaraj, M. (2020). GC-MS analysis of bioactive compounds from ethanolic extract of whole plant of Mollugo oppositifolia Linn and their pharmacological activities. International Journal of Pharmaceutical Sciences and Research, 11(5), 2504–2509.

    CAS  Google Scholar 

  3. Azhagumeena, C., & Bharathi, P. R. (2020). A review on phytochemistry and pharmacology of Calycopteris floribunda Lam. International Journal of Chemistry Research, 4(4), 1–8.

    Article  Google Scholar 

  4. Bhandary, M. J., Chandrashekar, K. R., & Kaveriappa, K. M. (1995). Medical ethnobotany of the Siddis of Uttara Kannada district, Karnataka, India. Journal of Ethnopharmacology, 47, 149–158.

    Article  CAS  PubMed  Google Scholar 

  5. Ratnagiriswaran, A. N., Sehra, K. B., & Venkataraman, K. (1934). The anthelminthic constituent of the leaves of Calycopteris floribunda Lam. Biochemical Journal, 28(6), 1964–1967.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Eswaraiah, M. C., & Satyanarayana, T. (2010). Hepatoprotective activity of extracts from stem of Calycopteris floribunda Lam. against carbon tetrachloride induced toxicity in rats. International Journal of Pharmacognosy and Phytochemical Research, 2(3), 53–57.

    Google Scholar 

  7. Pavithra, G. M., Naik, A. S., Siddiqua, S., Vinayaka, K. S., Kekuda, P. T. R., & Mukunda, S. (2013). Antioxidant and antibacterial activity of flower of Calycopteris floribunda Lam., Humboldtia brunonis Wall and Kydia calycina Roxb. International Journal of Drug Development & Research, 5(2), 99–102.

    Google Scholar 

  8. Satyanarayana, T., Eswaraiah, M. C., Bharathi, A., & Srinivas, M. (2009). Anti –inflammatory activity of stem of Calycopteris floribunda Lam. Journal of Pharmacy and Chemistry, 3(2), 57–59.

    Google Scholar 

  9. Vinayaka, K. S., Kekuda, T. P., Shivakumar, B., & Shravanakumara, S. (2009). Anti-dermatophyte activity of methanol and aqueous extracts of Calycopteris floribunda Lam. leaves. Natural Products: An Indian Journal, 5, 215–219.

    CAS  Google Scholar 

  10. Ali, H. A., Chowdhury, A. A., Rahman, A. K., Borkowski, T., Nahar, L., & Sarker, S. D. (2008). Pachypodol, a flavonol from the leaves of Calycopteris floribunda, inhibits the growth of CaCo2 colon cancer cell line in vitro. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 22, 1684–1687.

    Article  CAS  Google Scholar 

  11. Wall, M. E., Wani, M. C., Fullas, F., Oswald, J. B., Brown, D. M., Santisuk, T., & Farnsworth, N. R. (1994). Plant antitumor agents. 31.1 The calycopterones, a new class of biflavonoids with novel cytotoxicity in a diverse panel of human tumor cell lines. Journal of Medicinal Chemistry, 37, 1465–1470.

    Article  CAS  PubMed  Google Scholar 

  12. Suttaratrungse, K., Chanhan, P., Kanokmedhakul, K., Moosophon, P., & Kanokmedhakul, S. (2015). Two new flavonols from Flower of Getonia floribunda Roxb. Phytochemistry Letters, 11, 316–319.

    Article  CAS  Google Scholar 

  13. Mayer, R. (1999). Calycopterones and calyflorenones, novel biflavonoids from Calycopteris floribunda Lam. Journal of natural products, 62(9), 1274–1278.

    Article  CAS  PubMed  Google Scholar 

  14. Liu, J. J., Yang, D. L., Zhang, Y., Yuan, Y., Cao, F. X., Zhao, J. M., & Peng, X. B. (2009). Chemical component and antimicrobial activity of volatile oil of Calycopteris floribunda. Journal of Central South University of Technology, 16, 0931–0935.

    Article  CAS  Google Scholar 

  15. Hegde, S., Jayaraj, M. and Bhandarkar, A. V. (2015). Pharmacognostic studies and preliminary phytochemical analysis of cold and hot extracts of leaf of Tinospora malabarica Miers - An important medicinal plant. International Journal of Pharmaceutical Sciences Review and Research, 34(2), 04, 19–25.

  16. Kokate, C. K., Purohot , A.P and Gokhale. S.B. (2014). Pharmacognosy 50th Ed. (7.16–7.19) Nirali publication.

  17. Banakar, P., & Jayaraj, M. (2017). Pharmacognosy, phytochemistry and GC-MS analysis of ethanolic stem extract of Waltheria indica L. - A potent medicinal plant. Journal of Biologically Active Products from Nature, 7(5), 369–378.

    Article  CAS  Google Scholar 

  18. Sethuraman, S. P., & Ramachandran, K. P. (2021). Chemical profiling of volatile bioactives in Luisia tenuifolia Blume successive extracts by GC–MS analysis. Applied Biochemistry and Biotechnology., 194(1), 84–98.

    Article  PubMed  Google Scholar 

  19. Nagannawar, A. G., & Jayaraj, M. (2018). Pharmacognosy and preliminary phytochemical investigation on Mollugo oppositifolia Linn, a traditional medicinal herb. Journal of Pharmacognosy and Phytochemistry, 7(4), 509–512.

    CAS  Google Scholar 

  20. Glory, A., Judin, J., Vasudevan, R., & Sumathi, P. (2016). Preliminary phytochemical content and antibacterial activity of Ukshi (Calycopteris floribunda Lam.) leaves. Journal of Medicinal Plants Studies, 4(2), 57–59.

    Google Scholar 

  21. Tulika, T., & Agarwal, M. (2017). Phytochemical screening and GC-MS analysis of bioactive constituents in the ethanolic extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms. Journal of Pharmacognosy and Phytochemistry, 6(1), 195–206.

    Google Scholar 

  22. Arora, S., & Meena, S. (2017). GC-MS Profiling of Ceropegia bulbosa Roxb. var. bulbosa, an endangered plant from Thar Desert, Rajasthan. The Pharma Innovation Journal, 6(11), 568–573.

    CAS  Google Scholar 

  23. Kilonzo, M., Rubanza, C., Richard, U., & Sangiwa, G. (2019). Antimicrobial activities and phytochemical analysis of extracts from Ormocarpum trichocarpum (Taub.) and Euclea divinorum (Hiern) used as traditional medicines in Tanzania. Tanzania Journal of Health Research, 21(2), 1–12.

    Article  Google Scholar 

  24. Beulah, G. G., Soris, P. T., & Mohan, V. R. (2018). GC-MS determination of bioactive compounds of Dendrophthoe falcata (L.F) Ettingsh: An epiphytic plant. International Journal of Health Sciences and Research, 8(11), 261–269.

    Google Scholar 

  25. Vats, S., & Gupta, T. (2017). Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India. Physiology and molecular biology of plants, 23(1), 239–248.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Sivaranjani, V., Malarvili, T., Suganthi, K., & Mahalakshmi, S. (2021). Determination of bioactive compounds in Ulva reticulata extract using GC-MS technique. International Journal of Modern Agriculture, 10(2), 3309–3314.

    Google Scholar 

  27. Mutaku, J. F., Many, M. C., Colin, I., Denefand, J. F., & Van den Hove, M. F. (1998). Antigoitrogenic effect of combined supplementation with dl-tocopherol, ascorbic acid and, carotene and of dl-tocopherol alone in the rat. Journal of Endocrinology, 156(3), 551–561.

    Article  CAS  PubMed  Google Scholar 

  28. Chiswick, M. L., Johnson, M., Woodhall, C., Gowland, M., Davies, J., Toner, N., & Sims, D. G. (1983). Protective effect of vitamin E (DL-alpha-tocopherol) against intraventricular haemorrhage in premature babies. British Medical Journal, 287, 81–84.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Delicia, A. B., & Shyam, K. V. (2018). GC-MS analysis of bioactive compounds and antimicrobial activity of Cryptococcus rajasthanensis KY627764 isolated from Bombyx mori gut. Microflora. International Journal Advanced Research, 6(3), 525–538.

    Article  Google Scholar 

  30. Fernández, M. A., de las Heras, B., Garcia, M. D., Sáenz, M. T., & Villar, A. (2001). New insights into the mechanism of action of the anti-inflammatory triterpene lupeol. Journal of Pharmacy and Pharmacology, 53, 1533–1539.

    Article  PubMed  Google Scholar 

  31. Faridha Begum, I., Mohankumar, R., Jeevan, M., & Ramani, K. (2016). GC–MS analysis of bio-active molecules derived from Paracoccus pantotrophus FMR19 and the antimicrobial activity against bacterial pathogens and MDROs. Indian journal of microbiology, 56(4), 426–432.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Banakar, P., & Jayaraj, M. (2018). GC-MS analysis of bioactive compounds from ethanolic leaf extract of Waltheria indica Linn. and their pharmacological activities. International Journal of Pharmaceutical Sciences and Research, 9(5), 2005–2010.

    CAS  Google Scholar 

  33. Reddy, G. J., Reddy, K. B., & Reddy, G. S. (2020). GC-MS analysis and in-vitro anti-diabetic activity of bioactive fractions of Feronia elephantum fruit. International Journal of Pharmaceutical Sciences and Research, 11(5), 2415–2424.

    CAS  Google Scholar 

  34. Abdelhamid, M. S., Kondratenko, E. I., & Lomteva, N. A. (2015). GC-MS analysis of phytocomponents in the ethanolic extract of Nelumbo nucifera Gaertn. seeds from Russia. Journal of Applied Pharmaceutical Science, 5(04), 115–118.

    Article  Google Scholar 

  35. Amudha, M., & Rani, S. (2014). Assessing the bioactive constituents of Cadaba fruticosa (L.) Druce through GC-MS. International Journal of Pharmacy and Pharmaceutical Sciences, 6(2), 383–385.

    Google Scholar 

  36. Wanga, G. F., Shia, L. P., Rena, Y. D., Liu, Q. F., Liu, H. F., Zhang, R. J., Lie, Z., Zhua, F. H., Hea, P. L., Tanga, W., Taoe, P. Z., Chuan, L. C., Zhao, W. M., & Zuo, J. P. (2009). Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro. Antiviral Research, 83, 186–190.

    Article  Google Scholar 

  37. Rajeswari, G., Murugan, M., & Mohan, V. R. (2012). GC-MS analysis of bioactive components of Hugonia mystax L. (Linaceae). Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3(4), 304–308.

    Google Scholar 

  38. Kalsoom, R., Haider, M. S., & Chohan, S. (2020). Phytochemical analysis and antifungal activity of some medicinal plants against Alternaria species isolated from onion. The Journal of Animal & Plant Sciences, 30(2), 454–460.

    CAS  Google Scholar 

  39. Khan, B., Jabeen, K., Kanwal, Q., & Iqbal, S. (2021). Phytochemical constituents of pencil tree (Euphorbia tirucalli L.) as antifungal agent against mango anthracnose disease. Applied Ecology and Environmental Research, 19(4), 2915–2928.

    Article  Google Scholar 

  40. Arora, S., & Kumar, G. (2018). Phytochemical screening of root, stem and leaves of Cenchrus biflorus Roxb. Journal of Pharmacognosy and Phytochemistry, 7(1), 1445–1450.

    CAS  Google Scholar 

  41. Xiu-Zhen, Y., Yao-Haur, K., Tsong-Jyh, L., To-Shii, S., Chung-Hsiung, C., Mcphail, D. R., Mcphail, A. T., & Lees, K. H. (1989). Cytotoxic components of Diospyros morrisiana Hance. Phytochemistry, 28(5), 1541–15431.

    Article  Google Scholar 

  42. Koudehi, M. F., Ardalan, A. A., & Zibaseresht, R. (2020). Chemical constituents of an Iranian grown Capsicum annuum L. and their cytotoxic activities evaluation. Organic and Medicinal Chemistry International Journal, 9(3), 148–154.

    Google Scholar 

  43. Nair, R. V., Jayasree, D. V., Biju, P. G., & Baby, S. (2020). Anti-inflammatory and anticancer activities of erythrodiol-3-acetate and 2, 4-di-tert-butylphenol isolated from Humboldtia unijuga Bedd. Natural Product Research, 34(16), 2319–2322.

    Article  CAS  PubMed  Google Scholar 

  44. Ofosu, F. K., Chelliah, R., Daliri, E. B. M., Saravanakumar, K., Wang, M. H., & Oh, D. H. (2020). Antibacterial activities of volatile compounds in cereals and cereal by-products. Journal of Food Processing and Preservation, 45(2), e15081.

    Google Scholar 

Download references

Acknowledgements

We would like to acknowledge the Chairman of the PG Department of Studies in Botany, Karnatak University, Dharwad and Backward Classes Welfare Department, State Government of Karnataka for the financial support to one of the authors and KFRI Trissur, Kerala for GC-MS analysis.

Author information

Authors and Affiliations

Authors

Contributions

MJ planned and designed experiments, reviewing and editing; SBY collection and processing of plant material, investigation, formal analysis, writing, original draft preparation. Both the authors approved the final version of the manuscript.

Corresponding author

Correspondence to Sunil B. Yarazari.

Ethics declarations

Ethical Approval

Not applicable.

Consent to Participate

The authors have agreed to participate in the publication of the paper.

Consent for Publication

All authors have agreed to publish the paper.

Consent of Interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yarazari, S.B., Jayaraj, M. GC–MS Analysis of Bioactive Compounds of Flower Extracts of Calycopteris floribunda Lam.: A Multi Potent Medicinal Plant. Appl Biochem Biotechnol 194, 5083–5099 (2022). https://doi.org/10.1007/s12010-022-03993-7

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-022-03993-7

Keywords

Navigation