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Sensitive and Enzyme-Free Pathogenic Bacteria Detection Through Self-Circulation of Molecular Beacon

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Abstract

This research exhibits the design of a feasible, enzyme-free and sensitive fluorescent sensing assay for the detection of Staphylococcus aureus (S. aureus), using self-circulation of molecular beacons. With protein A on S. aureus as identifying target, the capture probe binds on the surface of S. aureus based on interaction between its aptamer section and protein A. Recognition of protein A by aptamer section in capture probe leads to allosterism of capture probe, exposing initiator section to activate the following self-circulation. After multiple circulation-based signal amplification, the method exhibits a favorable detection sensitivity and shows a promising prospect for the keratitis-related pathogenic bacteria detection. The highlights of the sensing assay are as follows: (i) capture probe is designed with aptamer section which endows the method a high selectivity; (ii) signal of bacteria is converted to nucleic acid signal after recognition of target bacteria by capture probe; and (iii) high sensitivity of method is derived from the self-circulation process. Therefore, we believe that the strategy can provide a useful platform for target bacteria detection and thus contribute to the diagnosis of infectious diseases.

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All data generated and analyzed during this study are included in this article.

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Acknowledgements

The authors thank the financial and equipment support from Shengjing Hospital of China Medical University.

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Authors and Affiliations

  1. Department of Ophthalmology, Zhuji Affiliated Hospital of Shaoxing University, No .9 Jianmin Road Taozhu Street, Zhuji City, 311800, Zhejiang Province, China

    Nannan Dong, Jiawei Zhao, Guangming Zhao & Tiewei Wang

  2. Department of Ophthalmology, Shengjing Hospital of China Medical University, Shenyang City, 110004, Liaoning Province, China

    Ning Jiang

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  1. Nannan Dong
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  2. Ning Jiang
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  3. Jiawei Zhao
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  4. Guangming Zhao
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  5. Tiewei Wang
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Contributions

D.N. designed the strategy, completed the preparation of the research, and wrote the manuscript; J.N., Z.J., Z.G., and W.T. assisted data analysis.

Corresponding author

Correspondence to Nannan Dong.

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Dong, N., Jiang, N., Zhao, J. et al. Sensitive and Enzyme-Free Pathogenic Bacteria Detection Through Self-Circulation of Molecular Beacon. Appl Biochem Biotechnol 194, 3668–3676 (2022). https://doi.org/10.1007/s12010-022-03948-y

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  • DOI: https://doi.org/10.1007/s12010-022-03948-y

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