Abstract
Marine alkaline protease (MAP) fermentation is a complex multivariable, multi-coupled, and nonlinear process. Some unmeasured parameters will affect the quality of protease. Aiming at the problem that some parameters are difficult to be detected online, a soft sensing modeling method based on improved Krill Herd algorithm RBF neural network (LKH-RBFNN) is proposed in this paper. Based on the multi-parameter RBFNN model, the adaptive RBF neural network algorithm and control law are used to approximate the unknown parameters. The adaptive Levy flight strategy is used to improve the traditional Krill Herd algorithm, improve the global search ability of the algorithm, and avoid falling into local optimization. At the same time, the location update formula of Krill Herd algorithm is improved by using the calculation methods of similarity and agglomeration degree, and the parameters of adaptive RBFNN are optimized to improve its over correction and large amount of calculation. Finally, the soft sensing prediction model of bacterial concentration and relative active enzyme in map process based on LKH-RBFNN is established. The root mean square error and maximum absolute error of this model are 0.938 and 0.569, respectively, which are less than KH-RBFNN and PSO-RBFNN prediction models. It proves that the prediction error of LKH-RBFNN model is smaller and can meet the needs of online prediction of key parameters of map fermentation.
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Acknowledgements
This study was supported by the Zhenjiang Key R&D Project (SH2020005) and Natural Science Foundation of Jiangsu Province (BK20191225).
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Hongyu Tang: Algorithm (LKH-RBFNN) design involved in system scheme. Feng Xu: Review of the paper (Part I: Introduction). Zhenli Yang: Algorithm simulation (Part III: Results and Discussion). Qi Wang: Selection of auxiliary parameters. Bo Wang: Analysis of on-line detection algorithm of bacterial concentration in marine alkaline protease (MAP) fermentation.
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Tang, H., Yang, Z., Xu, F. et al. Soft Sensor Modeling Method Based on Improved KH-RBF Neural Network Bacteria Concentration in Marine Alkaline Protease Fermentation Process. Appl Biochem Biotechnol 194, 4530–4545 (2022). https://doi.org/10.1007/s12010-022-03934-4
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DOI: https://doi.org/10.1007/s12010-022-03934-4