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Isolation and Phytochemical Screening of Endophytic Fungi Isolated from Medicinal Plant Mappia foetida and Evaluation of Its In Vitro Cytotoxicity in Cancer

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Abstract

Isolated endophyte fungi from Mappia foetida have been explored as a potential source for the mass production of anticancer drug lead compounds in the current study. Since medical plants are not feasible economically for mass production of bioactive pharmaceutical important molecules using plant tissue culture due to factors like media design and fungal contamination, endophyte fungal mass culture have been an alternative for the relatively easy and inexpensive production. Two endophytic fungi isolated, Alternaria alternata and Fusarium species were mass cultured and their prepared alcoholic extract subjected to standard procedures to identify the phytochemical screening by gas chromatography-mass spectrometry (GCMS), high-performance liquid chromatography (HPLC), UV visible spectrophotometry (UV–VIS), and Fourier transform infrared spectroscopy (FTIR). GC–MS analysis revealed the presence of three major compounds in the extracts. The phytochemical screening confirmed the presence of an anticancer compound (camptothecin) in their extract. Moreover, the dose-dependent anticancer activity of ethanol extract was demonstrated against cervical carcinoma (HeLa), breast carcinoma (MCF-7), non-small cell lung carcinoma (H1975), and hepatocellular carcinoma cell line (Hep G2) by MTT assay where doxorubicin was used as the positive control. Furthermore, the microscopic examination also confirmed the cytotoxic effect of extract of endophytic fungi Alternaria alternata and Fusarium species against tested cancer cells. Hence, endophytic fungi Alternaria alternata and Fusarium species might be exploited for mass production of phytochemicals having anticancer activity.

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Authors and Affiliations

  1. Department of Biotechnology & Bioinformatics, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga Dist., Karnataka, India, 577 451

    Pooja Ravi, Yerappa Lakshmikanth Ramachandra, Sunitha Bommanahalli Rudraiah & Mona Isaq

  2. School of Chemical Engineering, Yeungnam University, 280 Daehak-ro, Gyeongsan, 38541, Republic of Korea

    Prathap Somu & Yong Rok Lee

  3. Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (Deemed to Be University), Saveetha Nagar, Thandalam, Chennai, 602105, India

    Prathap Somu

  4. Department of Biotechnology, GIET University, Gunupur, Odisha, India, 765022

    Diptikanta Acharya

  5. Department of Biotechnology, Karunya Institute of Technology and Sciences (Deemed to Be University), Karunya Nagar, Coimbatore, Tamil Nadu, 641114, India

    Levin Anbu Gomez & Jesse Joel Thathapudi

  6. Department of Chemical Engineering, National Institute of Technology, Rourkela, Odisha, India

    Chetan Shekhar Karua

  7. Department of Agriculture Science, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan Campus Jeli, Locked Bag 100, Jeli, 17600, Kelantan, Malaysia

    Mohammed Arifullah

  8. Department of Biotechnology, Shridevi Institute of Engineering & Technology, Sira Road, Tumkur, Karnataka, India, 572106

    Chandrappa Chinna Poojari

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  1. Pooja Ravi
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Contributions

Conceptualization: Prathap Somu (Lead), Yerappa Lakshmikanth Ramachandra, Chandrappa Chinna Poojari, Yong Rok Lee.

Data collection, data analysis, and interpretation: Pooja Ravi, Diptikanta Acharya, Chetan Shekhar Karua, Mona Isaq, Jesse Joel Thathapudi, Mohammed Arifullah.

Drafting the article: Pooja Ravi, Levin Anbu Gomez, Sunitha Bommanahalli Rudraiah, Jesse Joel Thathapudi,

Critical revision of the article and approved: Prathap Somu, and Yong Rok Lee.

Corresponding authors

Correspondence to Prathap Somu, Chandrappa Chinna Poojari or Yong Rok Lee.

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Ravi, P., Somu, P., Acharya, D. et al. Isolation and Phytochemical Screening of Endophytic Fungi Isolated from Medicinal Plant Mappia foetida and Evaluation of Its In Vitro Cytotoxicity in Cancer. Appl Biochem Biotechnol 194, 4570–4586 (2022). https://doi.org/10.1007/s12010-022-03929-1

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