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Mimusops elengi Flower-Mediated Green Silver Nanoparticles Control Staphylococcus aureus and Acinetobacter baumannii

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Abstract

The use of antibiotics against infection-causing bacteria is inactivated, due to the presence of antibiotic-degrading enzymes. These enzymes resist the action of antibiotics and make difficult for the already existing antibiotics to break the drug resistance. Hence, there is a need to formulate alternate therapies against the enzymes providing multidrug resistance to bacteria including Staphylococcus aureus and Acinetobacter baumannii. A silver nanoparticle coated with the natural compounds is a way of finding an alternative to the traditional antibiotics. Objective of the study was to identify and assess Mimusops elengi’s floral silver nanoparticles (MENP) for its effective antibacterial activity. The methods involved were in silico analysis involving screening by ADME; drug likeness and docking of ligand to the protein (CrtM and CTX-M-15); in vitro analysis which included silver nanoparticle preparation; characterization by UV–Vis spectrum; SEM, FT-IR, DLS, and antimicrobial assays which include agar well diffusion, minimum inhibitory concentration, minimum bactericidal concentration, and anti-biofilm assays. The compounds present in the flower extract were identified and their usability against the enzymes under study was assured by silico screening. The in vitro analysis furthermore supports through showing potent antibacterial activity of MENP against selected organisms. Hence both in silico and in vitro studies reveal that MENP can be used as an alternative to break multidrug resistance.

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Data Availability

Data will be available on request.

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Abbreviations

ADME:

Absorption, digestion, metabolism, and excretion

SEM:

Scanning electron microscopy

FT-IR:

Fourier transform infrared spectroscopy

DLS:

Dynamic light scattering

MENP:

Mimusops elengi Silver nanoparticles

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Acknowledgements

Authors are thankful to B.S. Abdur Rahman Institute of Science and Technology, Chennai for providing research facilities in school of life sciences. The authors also gratefully acknowledge the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi (Ref No. DST/WOS-B/2018/1583-HFN (G)) and ASEAN University network (AUN)/Southeast Asia Engineering Education Development Network (SEED)/Japan International Cooperation Agency (JICA) SPRAC (SN042/MI.KU/2020)

Funding

Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi (Ref No. DST/WOS-B/2018/1583-HFN (G)) and ASEAN University network (AUN)/Southeast Asia Engineering Education Development Network (SEED)/Japan International Cooperation Agency (JICA) SPRAC (SN042/MI.KU/2020).

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  1. School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, 48, Tamilnadu, India

    Charlz Nithin J, Ranjani S & Hemalatha S

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  1. Charlz Nithin J
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SH conceived and designed research. SR and CNJ conducted experiments. SH analyzed data. All authors wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Hemalatha S.

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Charlz Nithin J and Ranjani S contributed equally.

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J, C.N., S, R. & S, H. Mimusops elengi Flower-Mediated Green Silver Nanoparticles Control Staphylococcus aureus and Acinetobacter baumannii. Appl Biochem Biotechnol 194, 3066–3081 (2022). https://doi.org/10.1007/s12010-022-03882-z

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