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Two-in-One Surfactant Disinfectant Potential of Xylitol Dicaprylate and Dilaurate Esters Synthesized by Talaromyces thermophilus galactolipase for Cleaning Industries

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Abstract

Talaromyces thermophilus galactolipase (TTL) was found to produce alcohol sugar fatty acid diesters. The modulation of the solvent composition was used for the esterification reaction screening of diesters from xylitol and various fatty acids using the immobilized Talaromyces thermophilus galactolipase. The reactions were assessed by LC–MS analysis. The antimicrobial activity assay showed that both xylitol dicaprylate and xylitol dilaurate esters had more ability to inhibit the growth of several bacteria involved in surface contamination in the food industry. The xylitol dilaurate ester has the highest activity against Gram-positive strains with the lowest MIC values of 0.0016 and 0.005 mg mL−1 against Bacillus licheniformis and Staphylococcus aureus, respectively. Xylitol dicaprylate ester is more active against Gram-negative ones with significantly low MIC values of 0.25 and 0.4 mg mL−1 against Escherichia coli and Pseudomonas aeruginosa, respectively. The highest antifungal activity of the xylitol dicaprylate ester has been also proven, with a MIC value of 0.02 mg mL−1 against Penicillium occitanis and Fusarium solani. A better reduction in critical micelle concentrations and air–water surface tension were observed with these diesters compared to their corresponding monoesters in addition to their efficient emulsifying properties. The stability of these diesters in a liquid detergent formula after one year of storage was tested by a positive oil spreading assay and a retained antimicrobial activity. They exhibit a typical surfactant behavior with a two-in-one effect that can act as a detergent and a disinfectant with potential use in different cleaning processes.

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Acknowledgements

We are grateful to Miss Lobna Jlaiel for her technical assistance during LC-MS experiments.

Funding

This work was supported financially by “Ministry of Higher Education and Scientific Research of Tunisia” through a grant to Laboratory of Eukaryotes Molecular Biotechnology, Centre of Biotechnology of Sfax, University of Sfax, TUNISIA. Part of this research work is carried out under the MOBIDOC project funded by the European Union under the PASRI program and administered by the ANPR.

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  1. Laboratoire de Biotechnologie Moléculaire Des Eucaryotes, Centre de Biotechnologie de Sfax, Université de Sfax, BP “1177”, 3018, Sfax, Tunisia

    Rabaa Ben Ayed, Maha Bouzid, Ali Gargouri, Hafedh Belghith & Inès Belhaj

  2. Neodeme (NEO-DETergent-Messadine) Company, Zone Industrielle Messadine, Sousse, Tunisia

    Raja Zbidi & Mustapha Aouioua

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  1. Rabaa Ben Ayed
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  2. Maha Bouzid
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  5. Ali Gargouri
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Contributions

R. Ben Ayed re-performed the synthesis reactions and antimicrobial activity experiments, and contributed to the surface properties measurements. M. Bouzid performed the synthesis reactions and the antimicrobial activity experiments. R. Zbidi discussed the diesters incorporation in detergent formula experiment. M. Aouioua provided the materials for the diesters incorporation in detergent formula. A. Gargouri discussed the results and revised the manuscript. H. Belghith validated the results and revised the manuscript. I. Belhaj contributed to the central idea, analyzed most of the data, and supervised and wrote the initial draft and revised paper.

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Correspondence to Inès Belhaj.

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Ben Ayed, R., Bouzid, M., Zbidi, R. et al. Two-in-One Surfactant Disinfectant Potential of Xylitol Dicaprylate and Dilaurate Esters Synthesized by Talaromyces thermophilus galactolipase for Cleaning Industries. Appl Biochem Biotechnol 194, 2700–2719 (2022). https://doi.org/10.1007/s12010-022-03864-1

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