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Macrolactin Metabolite Production by Bacillus sp. ZJ318 Isolated from Marine Sediment

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Abstract

A total of 172 microbial strains were screened and isolated from Arctic Ocean marine sediments at a depth of 42 ~ 3,763 m. A microorganism with strong antibacterial activity against Staphylococcus aureus was identified as Bacillus sp. ZJ318 according to the results of 16S rDNA sequencing and phylogenetic tree analyses. Bioactivity-guided isolation of the new/novel metabolite in the ethyl acetate (EA) extract obtained from the fermentation broth of this strain was followed by chromatographic fractionation and subsequent HPLC purification, leading to the isolation of one known macrolactin. The chemical structure of the macrolactin, which indicated macrolactin J isolation from marine microorganisms for the first time, was assigned based on a high-resolution electrospray ionization mass spectrometer system (HR-EMI-MS), nuclear magnetic resonance (NMR) spectral analyses, and a literature review. To improve macrolactin J production, the corresponding effects of nitrogen sources were investigated, and (NH4)2SO4 was determined to produce the best effect. In addition, the optimal culture conditions were determined by an orthogonal experiment. Under these conditions, the yield of macrolactin J was increased to 2.41 mg/L, which was 2.2 times the original yield. This work lays a foundation for follow-up mechanistic and application research on macrolactin J.

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Acknowledgements

Authors are grateful to the key lab of marine bioactive substance and modern analytical technique, SOA (MBSMAT-2019-05) , and the Dalian High Level Talents Innovation Support Plan (2019CT09).

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  1. Liying Zhang and Meishu Jin contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biotechnology and Bioresources Utilization (Ministry of Education), College of Life Science, Dalian Minzu University, Dalian, China

    Liying Zhang, Meishu Jin, Xian Shi, Liming Jin, Xiyan Hou, Baoquan Liu, Jijuan Cao & Chunshan Quan

  2. Polar Research Institute of China, Shanghai, China

    Yong Yu

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  1. Liying Zhang
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Correspondence to Chunshan Quan.

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Zhang, L., Jin, M., Shi, X. et al. Macrolactin Metabolite Production by Bacillus sp. ZJ318 Isolated from Marine Sediment. Appl Biochem Biotechnol 194, 2581–2593 (2022). https://doi.org/10.1007/s12010-022-03841-8

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