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Transcriptomic Analysis of Morphology Regulatory Mechanisms of Microparticles to Paraisaria dubia in Submerged Fermentation

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Abstract

Liquid submerged fermentation is an effective strategy to achieve large-scale production of active ingredients by macrofungi, and controlling mycelium morphology is a key factor restricting the development of this technology. Mining for superior morphological regulatory factors and elucidation of their regulatory mechanisms are vital for the further development of macrofungal fermentation technology. In this study, microparticles were used to control the morphology of Paraisaria dubia (P. dubia) in submerged fermentation, and the underlying regulatory mechanisms were revealed by transcriptomic. The relative frequency of S-type pellet diameter increased significantly from 7.14 to 88.31%, and biomass increased 1.54 times when 15 g/L talc was added. Transcriptome analysis showed that the morphological regulation of filamentous fungi was a complex biological process, which involved signal transduction, mycelium polar growth, cell wall synthesis and cell division, etc. It also showed a positive impact on the basic and secondary metabolism of P. dubia. We provided a theoretical basis for controlling the mycelium morphology of P. dubia in submerged fermentation, which will promote the development of macrofungal fermentation technology.

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All data generated or analyzed during this study are included in this published article and its additional files.

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Funding

This work was supported by the Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project TSBICIP-PTJS-003-04 and Natural Science Foundation of Jiangsu Province, China (Grant No. BK20200732) .

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  1. Ling-Ling tong and  Yue Wang are co-first authors.

Authors and Affiliations

  1. School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, No. 1, Wenyuan Road, Nanjing, 210023, People’s Republic of China

    Ling-Ling Tong, Yue Wang, Yuan-Hang Du, Li Yuan, Meng-Zhen Liu, Xin-Ya Mu, Zi-Lei Chen, Yi-Dan Zhang, Shao-Jie He, Xiu-Juan Li & Dong-Sheng Guo

  2. School of Biological and Chemical Engineering, Nanyang Institute of Technology, No. 80, Changjiang Road, Nanyang, 210023, People’s Republic of China

    Dong-Sheng Guo

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  1. Ling-Ling Tong
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Contributions

TLL and WY conceived and designed the experiments. TLL performed the experiments. WY, DYH, YL, LMZ, MXY, CZL, ZYD, and HSJ analyzed the data. TLL and WY wrote the manuscript with contributions from all authors. GDS and LXJ supervised the whole research work and revised the manuscript. All authors read and comment on the manuscript before submission.

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Correspondence to Xiu-Juan Li or Dong-Sheng Guo.

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Tong, LL., Wang, Y., Du, YH. et al. Transcriptomic Analysis of Morphology Regulatory Mechanisms of Microparticles to Paraisaria dubia in Submerged Fermentation. Appl Biochem Biotechnol 194, 4333–4347 (2022). https://doi.org/10.1007/s12010-022-03820-z

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