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Myrobalan-Mediated Nanocolloids in Controlling Marine Pathogens

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Abstract

Aquaculture production is affected by disease outbreak, which affects the production, profitability, and sustainability of the global aquaculture industry. Antibiotics have been widely used to control various infectious diseases. Indiscriminate usage of antibiotics results in development of antibiotic resistance in pathogens. This current study aims to synthesize myrobalan-mediated green silver nanocolloids (MBNc) by using the extract of three myrobalans and characterized by using various physiochemical techniques. Antibacterial potential of MBNc was screened in vibriosis causing pathogens (V. harveyi, V. alginolyticus, V. Parahaemolyticus), and foodborne pathogen S. haemolyticus, isolated from infected fish. Further, the presence of ESBL genes including CTX-M-15 and Amp C was analyzed in control and MBNc-treated strains. From our studies, it was observed that MBNc was very effective in controlling the growth. MBNc confirmed the anti-biofilm property in all tested marine pathogens and effectively abolish the genes encoding CTX-M-15 in tested pathogens. Thus, MBNc can be formulated to control the growth of marine pathogens and it can be used as an alternative to antibiotics to prevent infection in cage culturing and aquafarming.

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Acknowledgements

The authors are thankful to B.S. Abdur Rahman Institute of Science & Technology, Chennai, for providing research facilities in school of life sciences.

Funding

The authors gratefully acknowledge the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi. (Ref No. DST/WOS-B/2018/1583-HFN (G)) and ASEAN University network (AUN)/Southeast Asia Engineering Education Development Network (SEED)/Japan International Cooperation Agency (JICA) SPRAC (SN042/MI.KU/2020).

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Authors and Affiliations

  1. School of Life Sciences, B. S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, 600048, India

    S. Ranjani, Pradeep Parthasarathy & S. Hemalatha

  2. Central Marine Fisheries Research Institute, Mandapam, India

    P. Rameshkumar

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  1. S. Ranjani
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  2. Pradeep Parthasarathy
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  3. P. Rameshkumar
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  4. S. Hemalatha
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Contributions

SH conceived and designed research. SR and PP conducted experiments. PR contributed pathogenic strains. SH and PR analyzed data. All authors wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to S. Hemalatha.

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Ranjani, S., Parthasarathy, P., Rameshkumar, P. et al. Myrobalan-Mediated Nanocolloids in Controlling Marine Pathogens. Appl Biochem Biotechnol 194, 1120–1135 (2022). https://doi.org/10.1007/s12010-022-03816-9

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