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A Simple Fluorescent Aptasensing Platform Based on Graphene Oxide for Dopamine Determination

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Abstract

Dopamine (DA) is a catecholamine neurotransmitter playing an important role in different biological functions including central nervous, renal, cardiovascular, and hormonal systems. The sensitive and selective detection of this neurotransmitter plays a key role in the early diagnosis of various diseases related to abnormal levels of dopamine. Therefore, it is of great importance to explore rapid, simple, and accurate methods for detection of dopamine with high sensitivity and specificity. We propose in this work a fluorescent aptasensor based on graphene oxide (GO) as a quencher, for the rapid determination of dopamine. The principle of this aptasensor is based on fluorescence resonance energy transfer (FRET), where GO was used as energy donor, and a carboxy fluorescein (FAM)-labeled aptamer as acceptor. In the absence of DA, FAM-aptamer was adsorbed on the surface of GO through π-π stacking interactions between nucleotide bases and the carbon network, leading to a weak FRET and a quenching of the FAM fluorescence. However, by adding the target, the aptamer undergoes a conformational change to bind to DA with high affinity, resulting in a fluorescence recovery. Under the optimal experimental conditions, the fluorescence recovery was linearly proportional to the concentration of DA in the range of 3–1680 nM, with a limit of detection of 0.031 nM and a limit of quantification of 0.1 nM. Moreover, the developed assay exhibited minor response in the presence of various interferents and it revealed a satisfactory applicability in human serum samples.

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Acknowledgements

This work was financially supported by Bioengineering Laboratory, Higher National School of Biotechnology, Constantine, Algeria. We thank the National Biotechnology Research Center for providing the microplate reader.

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Authors and Affiliations

  1. Bioengineering Laboratory, Higher National School of Biotechnology, Constantine, Algeria

    Ahlem Teniou & Amina Rhouati

  2. BAE Laboratory, Perpignan University, Perpignan, France

    Gaëlle Catanante

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  1. Ahlem Teniou
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  2. Amina Rhouati
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Correspondence to Amina Rhouati.

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Teniou, A., Rhouati, A. & Catanante, G. A Simple Fluorescent Aptasensing Platform Based on Graphene Oxide for Dopamine Determination. Appl Biochem Biotechnol 194, 1925–1937 (2022). https://doi.org/10.1007/s12010-022-03802-1

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