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Development of Plant-Based Larvicide and Herbal Mosquito Repellent Fast Card with Reference to Identification of the Functional Bioactive Compounds Effective Against Culex Mosquito

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Abstract

Warm and humid climate creates ideal conditions for mosquito breeding. The ability of these vectors to spread a number of diseases to humans causes millions of deaths every year. Indiscriminate use of synthetic insecticides leads to the development of resistance in vector mosquitoes and along with this, these pesticides cause biological magnification of toxic components and affects adversely the non-target organisms including human being. Commercially available, chemically manufactured mosquito repellent fast cards are convenient to use and quite effective but burning of such card generates a lot of smoke and might be hazardous to human health in the long run. Thus, alternative approaches are to be adopted to control the population load of vector mosquito. Like that, the present study also reveals the larvicidal effect of Duranta leaf extract against Culex mosquito. In the present study, mosquito repellent fast card has been developed by Duranta-algal mixture which has shown better result than commercially available fast card on the basis of mosquito mortality as well as the amount of gases emitted. Again, the ethanolic crude extract of Duranta leaves leads to 100% mortality of all instars (Culex pipiens) larvae at both 1000 ppm and 500 ppm concentration. Therefore, the active component of Duranta has also been investigated. In Duranta, highest area percentage and peak have been shown by propionic acid in the retention time 18.086 by GC–MS. So, it can be confirmed that the major active ingredient is propionic acid in Duranta which is responsible for the mosquitocidal properties. Occurrence of propionic acid in Duranta has also been confirmed by the HPLC analysis.

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All data and materials generated or analyzed during this study are included in this published article.

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Acknowledgements

The authors would like to convey wholehearted gratitude to Dr. Vansanglura, Principal, Serampore College for his motivation in this work. They are also indebted to Mr. Anit Kumar Chakroborty, Head, Department of Zoology, Serampore College, for his unceasing support and advice during the entire work. Authors are also thankful to Prof. Nimai Chandra Saha, the Hon’ble Vice Chancellor, The University of Burdwan and Prof. Asish Kr Panigrahi, Pro Vice Chancellor, The University of Burdwan, for their inspiration throughout this work and Dr Indrani Chandra, Head of the Department of Biotechnology, The University of Burdwan for her support to carry out this research and Prof. Jai Prakash Keshari, Department of Botany, The University of Burdwan for his guidance in the development of herbal anti-mosquito fast card.

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Authors and Affiliations

  1. Department of Zoology for UG and PG Studies, Serampore College (Under the University of Calcutta), Serampore, West Bengal, India

    Amit Chattopadhyay, Vikky Shaw, Subarna Ghosh & Pranab Kr Banerjee

  2. Department of Biotechnology, The University of Burdwan, Bardhaman, 713 104, West Bengal, India

    Piyali Mukherjee

Authors
  1. Amit Chattopadhyay
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  2. Vikky Shaw
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  3. Piyali Mukherjee
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  4. Subarna Ghosh
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  5. Pranab Kr Banerjee
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Contributions

Dr. Amit Chattopadhyay and Dr. Subarna Ghosh have developed and evaluated the efficacy of phytochemical as larvicide. Dr. Amit Chattopadhyay along with Vikky Shaw and Dr. Piyali Mukherjee has produced the herbal anti-mosquito fast card and Dr. Pranab Kr Banerjee has supervised the entire work and guided to prepare the manuscript.

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Correspondence to Amit Chattopadhyay.

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Chattopadhyay, A., Shaw, V., Mukherjee, P. et al. Development of Plant-Based Larvicide and Herbal Mosquito Repellent Fast Card with Reference to Identification of the Functional Bioactive Compounds Effective Against Culex Mosquito. Appl Biochem Biotechnol 194, 2419–2430 (2022). https://doi.org/10.1007/s12010-022-03798-8

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