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Analysis of Antibiofilm Activities of Bioactive Compounds from Honeyweed (Leonurus sibiricus) Against P. aeruginosa: an In Vitro and In Silico Approach

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Abstract

Leonurus sibiricus (Red verticilla, honeyweed) is a type of herbaceous plant predominantly found in Asian subcontinents as weed in crop fields and is widely used for treating diabetes, bronchitis, and menstrual irregularities. However, there is a dearth of study in the application of the plant phytocompounds for treating biofilm-associated chronic infections. The bioactive compounds mainly comprise of tri-terpenes, di-terpenes, phenolic acid, and flavonoids which may have potential role as antimicrobial and antibiofilm agents. Acute and chronic infection causing microbes usually form biofilm and develop virulence factors and antibiotic resistance through quorum sensing (QS). In this study, the bioactive compounds leosibirin, sibiricinone A, leosibirone A, leonotin, quercetin, lavandulifolioside, and myricetin were identified using GC–MS analysis. These were used for analyzing the antibiofilm and anti-quorum sensing activities (rhamnolipid, AHL assay, swarming motility assay) against the biofilm formed by Pseudomonas aeruginosa, the most significant nosocomial disease-causing bacteria. The compounds were able to bring about maximum inhibition in biofilm formation and QS. Although the antibiofilm activity of the phytoextract was found to be higher than that of individual phytocompounds at a concentration of 250 µg/mL, quercetin and myricetin showed highest antibiofilm activity against Pseudomonas aeruginosa, respectively, at MIC values of 135 µg/mL and 150 µg/mL against P aeruginosa. FT-IR study also revealed that the active ingredients were able to bring about the destruction of exopolysaccharides (EPS). These observations were further validated by molecular docking interactions that showed the active ingredients inhibit the functioning of QS sensing proteins by binding with them. It was observed that myricetin showed better interactions with the QS proteins of P. aeruginosa. Myricetin and quercetin show considerable inhibition of biofilm in comparison to the phytocompounds. Thus, the present study suggests that the active compounds from L. sibiricus can be used as an alternate strategy in inhibiting the biofilm formed by pathogenic organisms.

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Author notes

  1. Sreejita Ghosh, Dibyajit Lahiri, Moupriya Nag and Ankita Dey contributed equally to this work.

Authors and Affiliations

  1. Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata, West Bengal, India

    Sreejita Ghosh, Ankita Dey, Bandita Dutta & Rina Rani Ray

  2. Department of Biotechnology, University of Engineering & Management, Kolkata, West Bengal, India

    Dibyajit Lahiri, Moupriya Nag, Riya Biswas & Dipro Mukherjee

  3. Department of Food Technology and Bio-Chemical Engineering, Jadavpur University, Kolkata, 700032, India

    Tanmay Sarkar

  4. Malda Polytechnic, West Bengal State Council of Technical Education, Government of West Bengal, Malda, 732102, India

    Tanmay Sarkar

  5. SIAN Institute, Association for Biodiversity Conservation and Research (ABC), 756001, Odisha, India

    Siddhartha Pati

  6. Department of Biotechnology, Academy of Management and Information Technology, Khordha, 752057, Odisha, India

    Siddhartha Pati

  7. Department of Biotechnology & Bioinformatics, Sambalpur University, Odisha, India

    Smaranika Pattanaik

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  1. Sreejita Ghosh
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The authors contributed equally.

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Correspondence to Rina Rani Ray.

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Ghosh, S., Lahiri, D., Nag, M. et al. Analysis of Antibiofilm Activities of Bioactive Compounds from Honeyweed (Leonurus sibiricus) Against P. aeruginosa: an In Vitro and In Silico Approach. Appl Biochem Biotechnol 195, 5312–5328 (2023). https://doi.org/10.1007/s12010-021-03797-1

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