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Bi-layered disulfiram-loaded fiber membranes with antibacterial properties for wound dressing

Abstract

In this study, the bi-layered disulfiram-loaded fiber membranes with the antibacterial activity and different surface wettabilities are prepared using electrospinning technology. In the application of wound dressing, the hydrophilic surface of fiber membranes is beneficial for cell adhesion and drug release to heal the wound. Meanwhile, the outside hydrophobic surface is able to block water penetration to reduce the probability of wound infection. The obtained bi-layered drug-loaded fiber membranes are composed of polyvinylidene fluoride (PVDF) bottom surface and disulfiram (DSF)/polylactic acid (PLA) top surface. To modify the top surface wettability, the oxygen plasma modification of bi-layered membranes was carried out. The morphology, wettability, and chemical compositions of bi-layered drug-loaded fiber membranes were analyzed using the scanning electronic microscope (SEM), drop shape analysis instrument, X-ray diffractometer (XRD), and X-ray photoelectron spectrometer (XPS). The bi-layered disulfiram-loaded membranes showed the potent antibacterial activity in vitro against both Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). It was found that the bi-layered membranes had good biocompatibility with L929 cells. Thus, the obtained bi-layered disulfiram-loaded fiber membranes are suitable for wound dressing application.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by the National Key R&D Program of China (No. 2017YFE0112100), EU H2020 Program (MNR4SCell No. 734174; NanoStencil No. 767285), Jilin Provincial Science and Technology Program (Nos. 20180414002GH, 20180414081GH, 20180520203JH, 20190702002GH, and 20200901011SF), Jilin Provincial DRC Research and Development Program (No. 2020C022-1), and “111” Project of China (No. D17017).

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Authors

Contributions

C. Xie contributed to the central idea, analyzed most of the data, and wrote the initial draft of the paper. J. Yan analyzed the XPS data. S. Cao performed the antibacterial activity experiment. R. Liu performed the surface wettability measurement. B. Sun analyzed the XRD data. Y. Xie assisted the surface wettability measurement. K. Qu provided the materials for antibacterial activity experiment. W. Zhang provided the majority of materials, reagents. Z. Weng contributed to refining the ideas and revised the manuscript. Z. Wang discussed the results and revised the manuscript.

Corresponding author

Correspondence to Zuobin Wang.

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All analyses were based on previously published studies; thus, no ethical approval and patient consent are required.

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Xie, C., Yan, J., Cao, S. et al. Bi-layered disulfiram-loaded fiber membranes with antibacterial properties for wound dressing. Appl Biochem Biotechnol 194, 1359–1372 (2022). https://doi.org/10.1007/s12010-021-03663-0

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  • DOI: https://doi.org/10.1007/s12010-021-03663-0

Keywords

  • Bi-layered disulfiram-loaded fiber membranes
  • Antibacterial activity
  • Surface wettability
  • Electrospinning
  • Wound dressing