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In Situ Calb Enzyme Immobilization in Mesoporous Material Type MCM-48 Synthesis Using Ionic Solid [C14MI]Cl as Structure-Directing Agent

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Abstract

MCM-48 mesoporous support was synthesized with the ionic solid 1-tetradecyl-3-methylimidazolium chloride ([C14MI]Cl) as a structure-directing agent for in situ immobilization of Candida antarctica B (CALB). The MCM-48[C14MI]Cl support showed characteristics of mesoporous material of interest, with a pore size of 20.30 and 73.41 A for the support without and with the enzyme, respectively. The elongation of the carbonic chain of the ionic solid directly influenced the increase in the specific area and pore volume of the material. In addition, the decrease in the specific area and pore volume for support with the enzyme showed the effectiveness of immobilization in situ. It was possible to obtain the ideal levels for the best activities of esterification of the enzyme with optimization of a mathematical model. The optimized variables were 0.31 g of enzyme and 3.35% of ionic solid with a maximum esterification activity of 392.92 U/g and 688% of yield. The support showed residual activity above 50% when stored under refrigeration for 75 days. At 60 and 80 °C, the enzyme immobilized on the support retained more than 80 and 40% of its residual activity, respectively. In addition, the support presented the possibility of reuse for up to 10 cycles with residual activity of approximately 50%. The support synthesized in the present study presents a great industrial opportunity for the immobilization and use of the CALB enzyme.

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Availability of Data and Materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank URI Erechim, National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and Research Support Foundation of the State of Rio Grande do Sul (FAPERGS).

Funding

This work was financially supported by CAPES, FAPERGS, and CNPq.

Author information

Authors and Affiliations

  1. IFRS – Erechim, Erechim, RS, 99713-028, Brazil

    Catia S. Zanchett Battiston

  2. Department of Food and Chemical Engineering, URI – Erechim, 1621, Sete de Setembro Av., Erechim, RS, 99709-910, Brazil

    Catia S. Zanchett Battiston, Aline M. Moreira Ficanha, Carolina E. Demaman Oro, Rogério Marcos Dallago & Marcelo Luis Mignoni

  3. Department of Engineering, Centro de Ensino Riograndense, Marau, RS, CEP 99150000, Brazil

    Aline M. Moreira Ficanha

Authors
  1. Catia S. Zanchett Battiston
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  2. Aline M. Moreira Ficanha
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  3. Carolina E. Demaman Oro
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  4. Rogério Marcos Dallago
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  5. Marcelo Luis Mignoni
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Contributions

C.S.Z.B: Investigation and writing.

A.M.M. Ficanha: Investigation and writing.

C.E.D. Oro: Investigation and writing.

R.M. Dallago: Formal analysis, writing, review, and editing.

M.L. Mignoni: Supervision, formal analysis, writing, review, and editing.

Corresponding author

Correspondence to Marcelo Luis Mignoni.

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All authors gave their consent to participate in the article (Marcelo L. Mignoni, on behalf of all co-authors).

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All authors gave their consent for publication of the article if it was accepted. (Marcelo L. Mignoni, on behalf of all co-authors).

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The authors declare no competing interests.

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Highlights

• MCM-48 was successfully synthesized using [C14MI]Cl, and CALB was immobilized.

• CALB immobilized in MCM-48 with [C14MI]Cl showed reuse for up to 10 cycles.

• The immobilization yield achieved was 688%.

• Enzymatic activity remained above 50% for 75 days under refrigeration.

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Battiston, C.S.Z., Ficanha, A.M.M., Oro, C.E.D. et al. In Situ Calb Enzyme Immobilization in Mesoporous Material Type MCM-48 Synthesis Using Ionic Solid [C14MI]Cl as Structure-Directing Agent. Appl Biochem Biotechnol 194, 748–761 (2022). https://doi.org/10.1007/s12010-021-03648-z

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