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Evaluation of Different Ionic Liquids as Additives in the Immobilization of Lipase CAL B by Sol-Gel Technique

Applied Biochemistry and Biotechnology volume 193, pages 2162–2181 (2021)Cite this article

Abstract

Sol-gel technique aiming enzymatic immobilization in situ with ionic liquids as additives is poorly studied. In this process, the addition of the enzyme is carried out in the synthesis of the support. The characteristics of ionic liquids, such as low vapor pressure, thermal stability, and non-flammability, make them strong candidates for use as immobilization additives. The objective of the present study was to immobilize the Candida antarctica B lipase by the sol-gel technique using ionic liquids as additives. The optimum points determined for ionic liquids 1-butyl-3-methylimidazolium chloride, 1-octyl-3-methylimidazolium bromide, and 1 hexadecyl-3-methylimimidazolium were 0.30, 0.27, and 0.22 g/mL of enzyme and 1.60, 1.52, and 1.52% of additive, respectively. The amount of enzyme and ionic liquids used in aerogel immobilization was the same as the optimized values in the xerogel immobilization process (for each ionic liquid). Ionic liquids proved to be good additives in the enzymatic immobilization process. Xerogel, regardless of the ionic liquid, presented a greater number of use cycles and better thermal stability compared to aerogel.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank URI-Erechim, National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and Research Support Foundation of the State of Rio Grande do Sul (FAPERGS).

Funding

This work was financially supported by CAPES, FAPERGS, and CNPq.

Author information

Affiliations

  1. Department of Engineering, Centro de Ensino Riograndense, Marau, RS, CEP 99150000, Brazil

    Aline Matuella Moreira Ficanha

  2. Department of Food and Chemical Engineering, URI – Erechim, 1621, Sete de Setembro Av, Erechim, RS, 99709-910, Brazil

    Aline Matuella Moreira Ficanha, Carolina Elisa Demaman Oro, Rogério Marcos Dallago & Marcelo Luis Mignoni

  3. Center for Studies on Colloidal Systems (NUESC)/Institute of Technology and Research (ITP), Postgraduate Programme in Industrial Biotechnology (PBI), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju, SE, CEP 49032-490, Brazil

    Elton Franceschi

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  1. Aline Matuella Moreira Ficanha
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  2. Carolina Elisa Demaman Oro
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  3. Elton Franceschi
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  4. Rogério Marcos Dallago
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  5. Marcelo Luis Mignoni
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Contributions

A.M.M. Ficanha: Investigation & Writing

C.E.D. Oro: Investigation & Writing

E. Franceschi: Formal Analysis, Writing, Review & Editing

R.M. Dallago: Formal Analysis, Writing, Review & Editing

M.L. Mignoni: Supervision, Formal Analysis, Writing, Review & Editing

Corresponding author

Correspondence to Aline Matuella Moreira Ficanha.

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Highlights

• Ionic liquids proved to be good additives in the process of immobilizing the CALB lipase in sol-gel.

• Among the ionic liquids used, C8 presented the best results.

• Xerogel showed a greater number of use cycles and better thermal stability compared to aerogel.

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Ficanha, A.M.M., Oro, C.E.D., Franceschi, E. et al. Evaluation of Different Ionic Liquids as Additives in the Immobilization of Lipase CAL B by Sol-Gel Technique. Appl Biochem Biotechnol 193, 2162–2181 (2021). https://doi.org/10.1007/s12010-021-03533-9

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  • DOI: https://doi.org/10.1007/s12010-021-03533-9

Keywords

  • Xerogel
  • Aerogel
  • Sol-gel technique
  • CALB
  • Ionic liquids