Effect of Different Pretreatment of Birch Sawdust on the Production of Active Polysaccharides by Inonotus obliquus Under Submerged Fermentation and Its Structural Mechanism

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Abstract

This study examined the effects of different pretreatments of birch sawdust on the production and activity of polysaccharides by Inonotus obliquus, and in order to explore the mechanism, structural characterization and analysis were carried out. The result clearly indicated that alkali treatment, ozone treatment, and alkali combined with ozone treatment of birch sawdust could be all helpful for the production of active polysaccharide by I. obliquus. Among four pretreatment groups, birch sawdust treated with alkali showed the highest increase in the exo-polysaccharide content (39.90%) and the inhibition rate of α-glucosidase (80.78%) within 11 days by the mycelium of I. obliquus through deep fermentation, in comparison to water-washed birch sawdust. Through a single-factor analysis and orthogonal experimental design, the optimum alkali treatment condition was as follows: NaOH concentration 1%, temperature 60 °C, and time 3 h. Moreover, the structural characteristics of pretreated birch sawdust with the optimum alkali treatment condition before and after fermentation by the mycelium of I. obliquus was performed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electronic microscopy. The results showed that alkali treatment destroyed the lignin structure of birch sawdust, exposed the cellulose in the amorphous area, reduced the crystallinity of lignocellulose, and damaged the surface structure of birch sawdust, which had a further damage and a greater degradation degree of birch sawdust after fermentation, indicating that alkali pretreatment was beneficial for utilization of birch sawdust by I. obliquus.

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Funding

This study received financial support from the National Natural Science Foundation of China (21576282).

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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Xiaohong Lu, Mengya Wang, and Zhezhen Zhao. The first draft of the manuscript was written by Xiaohong Lu. Ping Liu contributed to designing the experiments; Jinrong Hu and Jingsheng Zhang commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ping Liu.

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Lu, X., Wang, M., Zhao, Z. et al. Effect of Different Pretreatment of Birch Sawdust on the Production of Active Polysaccharides by Inonotus obliquus Under Submerged Fermentation and Its Structural Mechanism. Appl Biochem Biotechnol 193, 1545–1557 (2021). https://doi.org/10.1007/s12010-021-03508-w

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Keywords

  • Inonotus obliquus
  • Polysaccharide
  • α-Glucosidase
  • Birch sawdust
  • Pretreatment