Biochemical Properties of a Novel d-Mannose Isomerase from Pseudomonas syringae for d-Mannose Production

Abstract

d-Mannose isomerase can reversibly catalyze d-fructose to d-mannose which has various beneficial effects. A novel d-mannose isomerase gene (PsMIaseA) from Pseudomonas syringae was cloned and expressed in Escherichia coli. The recombinant d-mannose isomerase (PsMIaseA) showed the highest amino acid sequence homogeneity of 50% with ManI from Thermobifda fusca. PsMIaseA was purified through Ni-NTA chromatography, and its specific activity was 818.6 U mg–1. The optimal pH and temperature of PsMIaseA were pH 7.5 and 45 °C, respectively. The enzyme was stable within a wide pH range from 5.0 to 10.0. It could efficiently convert d-fructose to d-mannose without any metal ions. When PsMIaseA was incubated with 600 g/L d-fructose for 6 h, the space-time yield of d-mannose reached 27.2 g L–1 h–1 with a maximum conversion ratio of 27%. Therefore, the d-mannose isomerase may be suitable for green production of d-mannose.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 31901627), and the key state research and development plan “Modern Food Processing and Food Storage and Transportation Technology and Equipment” (No. 2016YFD0400804).

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Conceptualization, methodology, data curation, investigation, and writing—original draft preparation: Xiaohan Hua and Yanxiao Li. Conceptualization, methodology, and investigation: Junwen Ma. Conceptualization, supervision, and writing—review and editing: Zhengqiang Jiang. Supervision: Haijie Liu. Supervision and writing—review and editing: Qiaojuan Yan. All the authors read and approved the final manuscript.

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Correspondence to Qiaojuan Yan.

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Hua, X., Li, Y., Jiang, Z. et al. Biochemical Properties of a Novel d-Mannose Isomerase from Pseudomonas syringae for d-Mannose Production. Appl Biochem Biotechnol 193, 1482–1495 (2021). https://doi.org/10.1007/s12010-021-03487-y

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Keywords

  • Mannose
  • Mannose isomerase
  • Characterization
  • Pseudomonas syringae
  • Mannose production