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Engineering S. oneidensis for Performance Improvement of Microbial Fuel Cell—a Mini Review

Applied Biochemistry and Biotechnology volume 193, pages 1170–1186 (2021)Cite this article

Abstract

Microbial fuel cell (MFC) is a promising technology that utilizes exoelectrogens cultivated in the form of biofilm to generate power from various types of sources supplied. A metal-reducing pathway is utilized by these organisms to transfer electrons obtained from the metabolism of substrate from anaerobic respiration extracellularly. A widely established model organism that is capable of extracellular electron transfer (EET) is Shewanella oneidensis. This review highlights the strategies used in the transformation of S. oneidensis and the recent development of MFC in terms of intervention through genetic modifications. S. oneidensis was genetically engineered for several aims including the study on the underlying mechanisms of EET, and the enhancement of power generation and wastewater treating potential when used in an MFC. Through engineering S. oneidensis, genes responsible for EET are identified and strategies on enhancing the EET efficiency are studied. Overexpressing genes related to EET to enhance biofilm formation, mediator biosynthesis, and respiration appears as one of the common approaches.

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Authors and Affiliations

  1. Brain Research Institute Monash Sunway (BRIMS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University, Jalan Lagoon Selatan, Bandar Sunway, 47500, Subang Jaya, Selangor Darul Ehsan, Malaysia

    Dexter Hoi Long Leung

  2. Faculty of Science and Engineering, University of Nottingham, Broga Road, 43500, Semenyih, Selangor Darul Ehsan, Malaysia

    Yin Sze Lim & Siewhui Chong

  3. Department of Electro-Optical Engineering, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan

    Kasimayan Uma & Ja-Hon Lin

  4. Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan

    Guan-Ting Pan & Thomas Chung-Kuang Yang

Authors
  1. Dexter Hoi Long Leung
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  2. Yin Sze Lim
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  3. Kasimayan Uma
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  4. Guan-Ting Pan
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  5. Ja-Hon Lin
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  6. Siewhui Chong
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  7. Thomas Chung-Kuang Yang
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Contributions

Conceptualization and writing—original draft preparation: Dexter Hoi Long Leung; supervision: Yin Sze Lim; data analysis: Kasimayan Uma; resources: Ja-Hon Lin and Guan-Ting Pan; writing—review and editing: Siewhui Chong; funding acquisition: Thomas Chung-Kuang Yang.

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Correspondence to Siewhui Chong or Thomas Chung-Kuang Yang.

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Leung, D.H.L., Lim, Y.S., Uma, K. et al. Engineering S. oneidensis for Performance Improvement of Microbial Fuel Cell—a Mini Review. Appl Biochem Biotechnol 193, 1170–1186 (2021). https://doi.org/10.1007/s12010-020-03469-6

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Keywords

  • MFC
  • Shewanella
  • Microbial fuel cell
  • EET
  • Bioelectricity
  • Overexpressing