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High Gravity and Very High Gravity Fermentation of Sugarcane Molasses by Flocculating Saccharomyces cerevisiae: Experimental Investigation and Kinetic Modeling

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Abstract

Substantial progress has been made in ethanol fermentation technology under high gravity (HG) and very high gravity (VHG), which offer environmental and economic benefits. HG and VHG processes increase the productivity of ethanol, reduce distillation costs, and enable higher yields. The aim of the present study was to evaluate the use of sugarcane molasses as the medium component along with flocculating yeasts for fermentation in a fed-batch process employing this promising technology. We evaluated fed-batch fermentation, HG, and VHG involving a molasses-based medium with high concentrations of reducing sugars (209, 222, and 250 g/L). Fermentation of 222 g/L of total reducing sugars achieved 89.45% efficiency, with a final ethanol concentration of 104.4 g/L, whereas the highest productivity (2.98 g/(L.h)) was achieved with the fermentation of 209 g/L of total reducing sugars. The ethanol concentration achieved with the fermentation of 222 g/L of total reducing sugars was close to the value obtained for P’max (105.35 g/L). The kinetic model provided a good fit to the experimental data regarding the fermentation of 222 g/L. The results revealed that sugarcane molasses and flocculating yeasts can be efficiently used in HG fermentation to reduce the costs of the process and achieve high ethanol titers.

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Funding

The authors would like to acknowledge the Brazilian fostering agencies CAPES, CNPq, and FAPEMIG (TEC-APQ-02079-16 and TEC-APQ-02489-16) for financial support to this project.

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Authors and Affiliations

  1. Multidisciplinary Group of Research, Science and Technology (RMPCT), School of Chemical Engineering, Federal University of Uberlândia, Patos de Minas, MG, Brazil

    Cristiane Vieira Camargos, Vitória Demétrio Moraes, Liliane Maciel de Oliveira, Carla Zanella Guidini & Líbia Diniz Santos

  2. School of Chemical Engineering, Federal University of Uberlândia, Uberlândia, MG, Brazil

    Eloízio Júlio Ribeiro

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  1. Cristiane Vieira Camargos
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  2. Vitória Demétrio Moraes
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Camargos, C.V., Moraes, V.D., de Oliveira, L.M. et al. High Gravity and Very High Gravity Fermentation of Sugarcane Molasses by Flocculating Saccharomyces cerevisiae: Experimental Investigation and Kinetic Modeling. Appl Biochem Biotechnol 193, 807–821 (2021). https://doi.org/10.1007/s12010-020-03466-9

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