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Conservation of Xylose Fermentability in Phlebia Species and Direct Fermentation of Xylan by Selected Fungi

Abstract

In efforts to lower the cost of total conversion of lignocellulosic materials, utilization of hemicellulose must be considered. White-rot fungus Phlebia sp. MG-60 can produce ethanol directly from cellulose and has fermentation ability for glucose, cellulose, and xylose. Therefore, white-rot fungi can be considered a good candidate for consolidated bioprocessing to give bioethanol from lignocellulosic biomass, although little information is available on the direct fermentation of xylan. In the present study, some Phlebia species were selected as candidates because of their ability to ferment xylose to ethanol more efficiently than Phlebia sp. MG-60. This process indicated that the basidiomycetes that can produce ethanol from xylose are closely related genetically within the Phlebia genus. The selected Phlebia species showed higher ethanol productivity from corn core and beechwood xylans than Phlebia sp. MG-60. The ethanol yields from corn core xylan in culture with Phlebia acerina HHB11146, Phlebia ludoviciana HHB9640, and Phlebia subochracea HHB8494 were 46.2%, 46.7%, and 39.7% of theoretical maximum, and those from beechwood xylan were 19.09%, 17.7%, and 21.4% of the theoretical maximum, respectively.

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Acknowledgments

We thank Austin Schultz, PhD, from Edanz Group, (https://en-author-services.edanzgroup.com/) for editing a draft of this manuscript.

Funding

This work was supported by the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry (27006A) from the Ministry of Agriculture, Forestry, and Fisheries of Japan. This work was also supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant no. 18H02257 and 17K19296).

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Correspondence to Ichiro Kamei.

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Kamei, I., Uchida, K. & Ardianti, V. Conservation of Xylose Fermentability in Phlebia Species and Direct Fermentation of Xylan by Selected Fungi. Appl Biochem Biotechnol 192, 895–909 (2020). https://doi.org/10.1007/s12010-020-03375-x

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Keywords

  • Xylose fermentation
  • Xylan fermentation
  • Basidiomycetes
  • Phlebia genus
  • Bioethanol